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Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications
Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and vi...
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| Published in: | BMC research notes 2024-12, Vol.17 (1), p.389-7, Article 389 |
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| creator | Esemu, Seraphine Nkie Nya'Nying, Shantal Fuenbi Ndip, Lucy Mande Bessong, Pascal Obong Tanih, Nicoline Fri Smith, Stella Ifeanyi Ndip, Roland Ndip |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and virulence factors.
We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A
-A
) were identified and the most prevalent was A
(TMP
E
TET
AMP
P
). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms. |
| doi_str_mv | 10.1186/s13104-024-07073-3 |
| format | article |
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We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A
-A
) were identified and the most prevalent was A
(TMP
E
TET
AMP
P
). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.</description><identifier>ISSN: 1756-0500</identifier><identifier>EISSN: 1756-0500</identifier><identifier>DOI: 10.1186/s13104-024-07073-3</identifier><identifier>PMID: 39731175</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antimicrobial resistance ; Bovine mastitis ; Cameroon ; Cameroon - epidemiology ; Care and treatment ; Cattle ; Diagnosis ; Drug resistance in microorganisms ; Drug Resistance, Multiple, Bacterial - genetics ; Female ; Genotype ; Humans ; Mastitis ; Mastitis, Bovine - epidemiology ; Mastitis, Bovine - microbiology ; Methicillin-resistant Staphylococcus aureus ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Microbial Sensitivity Tests ; Milk - microbiology ; Milk contamination ; Prevention ; Public Health ; Staphylococcal Infections - epidemiology ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Virulence factors ; Virulence Factors - genetics</subject><ispartof>BMC research notes, 2024-12, Vol.17 (1), p.389-7, Article 389</ispartof><rights>2024. The Author(s).</rights><rights>COPYRIGHT 2024 BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c395t-21a503d1610b4628cdc40e3d84523c23b27435a4a53fc4044884401437d28aa53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902,36990</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39731175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Esemu, Seraphine Nkie</creatorcontrib><creatorcontrib>Nya'Nying, Shantal Fuenbi</creatorcontrib><creatorcontrib>Ndip, Lucy Mande</creatorcontrib><creatorcontrib>Bessong, Pascal Obong</creatorcontrib><creatorcontrib>Tanih, Nicoline Fri</creatorcontrib><creatorcontrib>Smith, Stella Ifeanyi</creatorcontrib><creatorcontrib>Ndip, Roland Ndip</creatorcontrib><title>Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications</title><title>BMC research notes</title><addtitle>BMC Res Notes</addtitle><description>Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and virulence factors.
We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A
-A
) were identified and the most prevalent was A
(TMP
E
TET
AMP
P
). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial resistance</subject><subject>Bovine mastitis</subject><subject>Cameroon</subject><subject>Cameroon - epidemiology</subject><subject>Care and treatment</subject><subject>Cattle</subject><subject>Diagnosis</subject><subject>Drug resistance in microorganisms</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Female</subject><subject>Genotype</subject><subject>Humans</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - epidemiology</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Methicillin-resistant Staphylococcus aureus</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - isolation & purification</subject><subject>Microbial Sensitivity Tests</subject><subject>Milk - microbiology</subject><subject>Milk contamination</subject><subject>Prevention</subject><subject>Public Health</subject><subject>Staphylococcal Infections - epidemiology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>Virulence factors</subject><subject>Virulence Factors - genetics</subject><issn>1756-0500</issn><issn>1756-0500</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNptkstu1TAQhiMEoqXwAiyQJTZlkeJr7LCrjrgcqaILbktr4jgnrpI42A6ivAfvi89JqTgSsqyxZ75_rLH-onhO8AUhqnodCSOYl5jmLbFkJXtQnBIpqhILjB_-cz4pnsR4g3FFlCKPixNWS0Zy9bT4vY1-gOT8hGBqkekhgEk2uF9r0ndotKl3xg2Dm8pgo4sJpoQ-JZj728Ebb8wSESzB5tAFP6LG_3CTRSPE5JKLyE3oow-pR99sTGgDow3eT2_QvDSDM6i3MOSiG-d8O7wanxaPOhiifXYXz4ov795-3nwor67fbzeXV6VhtUglJSAwa0lFcMMrqkxrOLasVVxQZihrqORMAAfBulzhXCnOMeFMtlRBzp4V27Vv6-FGz8GNEG61B6cPCR92GkJyZrBaEtMJTLsaG8kxiNoABmNANRIIUJJ7na-95uC_L3lSPbpo7DDAZP0SNSO8lkIJskdfrugOcmc3dT7lX9_j-lJRkiEuWaYu_kPl1drRGT_ZzuX8keDVkSAzyf5MO1hi1Nvrr8csXVkTfIzBdvfTE6z37tKru3R2lz64S-9FL-5GXJrRtveSv3ZifwAkEcpr</recordid><startdate>20241227</startdate><enddate>20241227</enddate><creator>Esemu, Seraphine Nkie</creator><creator>Nya'Nying, Shantal Fuenbi</creator><creator>Ndip, Lucy Mande</creator><creator>Bessong, Pascal Obong</creator><creator>Tanih, Nicoline Fri</creator><creator>Smith, Stella Ifeanyi</creator><creator>Ndip, Roland Ndip</creator><general>BioMed Central Ltd</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20241227</creationdate><title>Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications</title><author>Esemu, Seraphine Nkie ; Nya'Nying, Shantal Fuenbi ; Ndip, Lucy Mande ; Bessong, Pascal Obong ; Tanih, Nicoline Fri ; Smith, Stella Ifeanyi ; Ndip, Roland Ndip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-21a503d1610b4628cdc40e3d84523c23b27435a4a53fc4044884401437d28aa53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial resistance</topic><topic>Bovine mastitis</topic><topic>Cameroon</topic><topic>Cameroon - epidemiology</topic><topic>Care and treatment</topic><topic>Cattle</topic><topic>Diagnosis</topic><topic>Drug resistance in microorganisms</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Female</topic><topic>Genotype</topic><topic>Humans</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - epidemiology</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Methicillin-resistant Staphylococcus aureus</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - isolation & purification</topic><topic>Microbial Sensitivity Tests</topic><topic>Milk - microbiology</topic><topic>Milk contamination</topic><topic>Prevention</topic><topic>Public Health</topic><topic>Staphylococcal Infections - epidemiology</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Infections - veterinary</topic><topic>Virulence factors</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Esemu, Seraphine Nkie</creatorcontrib><creatorcontrib>Nya'Nying, Shantal Fuenbi</creatorcontrib><creatorcontrib>Ndip, Lucy Mande</creatorcontrib><creatorcontrib>Bessong, Pascal Obong</creatorcontrib><creatorcontrib>Tanih, Nicoline Fri</creatorcontrib><creatorcontrib>Smith, Stella Ifeanyi</creatorcontrib><creatorcontrib>Ndip, Roland Ndip</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints Resource Center</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>BMC research notes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Esemu, Seraphine Nkie</au><au>Nya'Nying, Shantal Fuenbi</au><au>Ndip, Lucy Mande</au><au>Bessong, Pascal Obong</au><au>Tanih, Nicoline Fri</au><au>Smith, Stella Ifeanyi</au><au>Ndip, Roland Ndip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications</atitle><jtitle>BMC research notes</jtitle><addtitle>BMC Res Notes</addtitle><date>2024-12-27</date><risdate>2024</risdate><volume>17</volume><issue>1</issue><spage>389</spage><epage>7</epage><pages>389-7</pages><artnum>389</artnum><issn>1756-0500</issn><eissn>1756-0500</eissn><abstract>Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen that poses a serious threat to veterinary and public health worldwide. We investigated mastitis milk samples for contamination with MRSA and also characterized the MRSA isolates by investigating antimicrobial resistance and virulence factors.
We confirmed MRSA in 69 of 201 (34.3%) S. aureus isolates recovered from a total of 300 samples. Of the 69 MRSA, 19 (27.5%) were from subclinical cases, while 50 (72.5%) were from clinical cases. The MRSA showed high resistance to penicillin (100%), ampicillin (100%), trimethoprim (69.6%), and tetracycline (69.6%) while susceptibility was observed for gentamicin (100%), vancomycin (95.7%), and ciprofloxacin (91.3%). Most isolates (65.2%, 45/69) were multidrug resistant. Thirteen antibiotypes (A
-A
) were identified and the most prevalent was A
(TMP
E
TET
AMP
P
). All MRSA produced haemolysins, caseinase, and coagulase. Lipase, gelatinase and lecithinase were found in 97.1%, 94.2% and 91.3% of isolates respectively. Genotyping revealed coa (100%) and spa (68.1%) genes. We recommend educating dairy farmers on the public health implications of consuming unpasteurized raw milk and the implementation of proper hygiene practices in dairy farms.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>39731175</pmid><doi>10.1186/s13104-024-07073-3</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | BMC research notes, 2024-12, Vol.17 (1), p.389-7, Article 389 |
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| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_71cf502f90c740a59ca0acca8b7a1a21 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Animals Anti-Bacterial Agents - pharmacology Antimicrobial resistance Bovine mastitis Cameroon Cameroon - epidemiology Care and treatment Cattle Diagnosis Drug resistance in microorganisms Drug Resistance, Multiple, Bacterial - genetics Female Genotype Humans Mastitis Mastitis, Bovine - epidemiology Mastitis, Bovine - microbiology Methicillin-resistant Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - isolation & purification Microbial Sensitivity Tests Milk - microbiology Milk contamination Prevention Public Health Staphylococcal Infections - epidemiology Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Virulence factors Virulence Factors - genetics |
| title | Isolation and characterization of methicillin-resistant Staphylococcus aureus from bovine mastitis in North West Cameroon: public health implications |
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