Systematic identification and functional characterization of the CFEM proteins in fishscale bamboo rhombic-spot pathogen Neostagonosporella sichuanensis

Fungal effectors play a crucial role in the interaction between pathogenic fungi and their hosts. These interactions directly influence the invasion and spread of pathogens, and the development of diseases. Common in fungal extracellular membrane (CFEM) effectors are closely associated with the path...

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Bibliographic Details
Published in:Frontiers in plant science 2024-05, Vol.15, p.1396273-1396273
Main Authors: Liang, Fang, Liu, Lijuan, Li, Chengsong, Liu, Yinggao, Han, Shan, Yang, Hua, Li, Shujiang, Hui, Wenkai, Liu, Long, Yang, Chunlin
Format: Article
Language:English
Subjects:
CFEM domain
effector
Neostagonosporella sichuanensis
plant immunity
Plant Science
stress tolerance
virulence
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Summary:Fungal effectors play a crucial role in the interaction between pathogenic fungi and their hosts. These interactions directly influence the invasion and spread of pathogens, and the development of diseases. Common in fungal extracellular membrane (CFEM) effectors are closely associated with the pathogenicity, cell wall stability, and pathogenic processes of pathogenic fungi. The aim of this study was to investigate the role of CFEM proteins in in pathogen-host interactions. We retrieved 19 proteins containing CFEM structural domains from the genome of . By systematic analysis, five NsCFEM proteins had signal peptides but lacked transmembrane structural domains, and thus were considered as potential effectors. Among them, NsCFEM1 and NsCFEM2 were successfully cloned and their functions were further investigated. The validation results show that NsCFEM1 was localized in the cell membrane and nucleus, whereas NsCFEM2 was exclusively observed in the cell membrane. Both were identified as secreted proteins. Additionally, NsCFEM1 inhibited Bax-induced programmed cell death in , whereas NsCFEM2 did not induce or inhibit this response. NsCFEM1 was implicated as a virulence factor that contributes to fungal growth, development, stress response, and pathogenicity. NsCFEM2 was implicated in maintenance of cell wall stability. This study lays a foundation for elucidating the role of CFEM proteins in the pathogen of fishscale bamboo rhombic-spot caused by . In particular, the functional studies of NsCFEM1 and NsCFEM2 revealed their potential roles in the interaction between and the host .
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2024.1396273