Production of exopolysaccharides from novel marine bacteria and anticancer activity against hepatocellular carcinoma cells (HepG2)

Background The aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells. Results Eleven isolates have the ability to produce EPSs and also...

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Published in:Bulletin of the National Research Centre 2018-12, Vol.42 (1), p.1-9, Article 30
Main Authors: Asker, Mohsen S., El Sayed, Osama H., Mahmoud, Manal G., Yahya, Shaymaa M., Mohamed, Sahar S., Selim, Manal S., El Awady, Mohamed S., Abdelnasser, Salma M., Abo Elsoud, Mostafa M.
Format: Article
Language:English
Subjects:
Anticancer properties
Antitumor activity
Bacteria
Chemical composition
Cytotoxicity
Exopolysaccharides
Galactose
Hepatocellular carcinoma
Hepatocellular carcinoma cells
Humanities and Social Sciences
Liver cancer
Mannose
Marine bacteria
Marine sediments
Molecular weight
multidisciplinary
Pseudomonas fluorescens
Ribonucleic acid
RNA
Science
Science (multidisciplinary)
Sulfates
Toxicity
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Summary:Background The aim of the current study based on the production and characterization of exopolysaccharides (EPSs) isolated from marine sediment of the Mediterranean and Red Seas is to study its cytotoxic activity against HepG2 cells. Results Eleven isolates have the ability to produce EPSs and also decreased the viability of HepG2 cell line in different manners. The five most promising isolates that produce high yield of EPSs and high cytotoxicity were identified by 16S RNA as Brevundimonas subvibrioides MSA1, Bacillus thuringiensis E4, Bacillus amyloliquefaciens MGA2, Pseudomonas fluorescens SGA3, and Advenella Kashmirensis NRC-7. The chemical composition of the following EPSs (M1, M3, M6, M15, M19, E2, E4, E10, S5, S7, and S11) demonstrates that they are acidic sulfated heteropolysaccharides with different relative ratios of monosugars of glucose, mannose, galactose, glucouronic acid, and mannouronic acid. The average molecular weights from 1.94 × 10 4 to 7.95 × 10 5  g/mol and the number average molecular weight from 1.51 × 10 4 to 7.53 × 10 5  g/mol. FTIR spectrum of the five EPSs indicated the presence of sulfate and carboxylic groups in different percentages. Conclusions The EPSs produced from marine bacteria are very promising for treating the HepG2 cells.
ISSN:2522-8307
2522-8307
DOI:10.1186/s42269-018-0032-3