Molecular Detection Of Multidrug-Resistant Salmonella Isolated From Livestock Production Systems In South Africa

• Published in: Infection and drug resistance 2019-11, Vol.12, p.3537-3548
• Main Authors: Mthembu, Thobeka P, Zishiri, Oliver T, El Zowalaty, Mohamed E
• Format: Article
• Language: English
• Subjects: Amoxicillin; Ampicillin; animals; Antibacterial agents; antimicrobial; Azithromycin; Bacteria; blacmy-2; blatem-1; Cattle; Chloramphenicol; Ciprofloxacin; Clavulanate; Disease transmission; DNA; Drug resistance; food-borne pathogens; Genes; Genetic research; Goats; Health aspects; Livestock; Livestock industry; Microbial drug resistance; Original Research; Pathogenic microorganisms; Penicillins; Polymerase chain reaction; Public health; Public health movements; resistance; Salmonella; Sustainable development; Tetracyclines; Zoonoses; zoonotic
• ISSN: 1178-6973; 1178-6973
• DOI: 10.2147/IDR.S211618

Antibiotic-resistant bacterial pathogens associated with livestock remain a major concern worldwide as they get transmitted from animals to humans and cause foodborne and zoonotic diseases. Antimicrobial resistance in livestock-associated spp in South Africa was investigated using molecular DNA methods. Three hundred and sixty-one environmental faecal samples were randomly collected from avian (chicken and ducks), cows, pigs, goats, and sheep. spp. were isolated on selective media and were confirmed using the polymerase chain reaction. Antimicrobial susceptibility testing against ampicillin, chloramphenicol, ciprofloxacin, ceftriaxone, azithromycin, tetracycline, amoxicillin-clavulanate and trimethoprim-sulfamethoxazole was determined using the Kirby-Bauer disk diffusion method. Isolates were screened for the presence of and resistance genes by PCR. Most of the isolates were resistant to ampicillin (64%), tetracycline (63%), amoxicillin-clavulanate (49%), trimethoprim-sulfamethoxazole (38%), and ceftriaxone (20%). Eight percent of the tested isolates were ciprofloxacin-resistant spp. Multidrug resistance was observed with the mean multiple antibiotic resistance (MAR) index of 0.31. The study demonstrated that 43% of the isolates were multiple drug resistant. The prevalence rates of resistance genes were 44% for , 35% for , 21% for , 18% for , 14% for and 8% for . Resistance to ceftriaxone, detection of gene and the high level of intermediate susceptibility (33%) against ciprofloxacin suggested that livestock carry problematic spp. This study used the global one-health initiative to report the potential public health risks of livestock-associated pathogens and highlights the importance of monitoring the trends of antimicrobial resistance for sustainability of antibiotics.