Loading…
hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein
Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) plays an important role in regulating the replication of many viruses. However, it remains elusive whether and how hnRNPA1 regulates fish virus replication. In this study, the effects of twelve hnRNPs on the replication of snakehead vesiculovirus...
Saved in:
Published in: | Virulence 2023-12, Vol.14 (1), p.2196847-2196847 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | cdi_FETCH-LOGICAL-c483t-45b6150b1c1bbcf4cc06b792a1bfb6eca794275decaef707ea9b28964009498d3 |
container_end_page | 2196847 |
container_issue | 1 |
container_start_page | 2196847 |
container_title | Virulence |
container_volume | 14 |
creator | Liu, An-Qi Qin, Xiangmou Wu, Hui Feng, Hao Zhang, Yong-An Tu, Jiagang |
description | Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) plays an important role in regulating the replication of many viruses. However, it remains elusive whether and how hnRNPA1 regulates fish virus replication. In this study, the effects of twelve hnRNPs on the replication of snakehead vesiculovirus (SHVV) were screened. Three hnRNPs, one of which was hnRNPA1, were identified as anti-SHVV factors. Further verification showed that knockdown of hnRNPA1 promoted, while overexpression of hnRNPA1 inhibited, SHVV replication. SHVV infection reduced the expression level of hnRNPA1 and induced the nucleocytoplasmic shuttling of hnRNPA1. Besides, we found that hnRNPA1 interacted with the viral phosphoprotein (P) via its glycine-rich domain, but not with the viral nucleoprotein (N) or large protein (L). The hnRNPA1-P interaction competitively disrupted the viral P-N interaction. Moreover, we found that overexpression of hnRNPA1 enhanced the polyubiquitination of the P protein and degraded it through proteasomal and lysosomal pathways. This study will help understanding the function of hnRNPA1 in the replication of single-stranded negative-sense RNA viruses and providing a novel antiviral target against fish rhabdoviruses. |
doi_str_mv | 10.1080/21505594.2023.2196847 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_72212d3bdb4b4a608ad34f93fa6da0e6</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_72212d3bdb4b4a608ad34f93fa6da0e6</doaj_id><sourcerecordid>2794685758</sourcerecordid><originalsourceid>FETCH-LOGICAL-c483t-45b6150b1c1bbcf4cc06b792a1bfb6eca794275decaef707ea9b28964009498d3</originalsourceid><addsrcrecordid>eNp9UsFu1DAQjRCIVqWfAMqRyy6248TJCaqq0EoVIARna2xPdl0SO9jOov0dvrTe7m5FD2DJ8njmvTce6xXFa0qWlLTkHaM1qeuOLxlh1ZLRrmm5eFac7vKLuiHt82OcQSfFeYx3JC_e0kx7WZxUgpBaCHpa_Fm7b5-_XtDSjhMajGV08BPXCKbcYLR6HvzGhjmWAafBakjWu3JjodQ-E5JNdoPDtjQ2hnlK1q1yMcBQTmsf856CT2jdws16wOOttC5hAP2gBc6UBlcBzL_Ir4oXPQwRzw_nWfHj49X3y-vF7ZdPN5cXtwvN2yoteK2aPLKimiqle641aZToGFDVqwY1iI4zUZscYS-IQOgUa7uGE9LxrjXVWXGz1zUe7uQU7AhhKz1Y-ZDwYSUhJJsHkYIxykyljOKKQ_5vMBXvu6qHxgDBJmu932tNsxrRaHQpD_ZE9GnF2bVc-Y2khAhGSZcV3h4Ugv81Y0xytFHjMIBDP0fJ8jhNW4u6zdB6D9XBxxiwf-xDidz5RR79Ind-kQe_ZN6bvx_5yDq6IwM-7AHW9T6M8NuHwcgE28GHPoDTNsrq_z3uAbSu1rI</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2794685758</pqid></control><display><type>article</type><title>hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein</title><source>PubMed (Medline)</source><source>Taylor & Francis Open Access</source><creator>Liu, An-Qi ; Qin, Xiangmou ; Wu, Hui ; Feng, Hao ; Zhang, Yong-An ; Tu, Jiagang</creator><creatorcontrib>Liu, An-Qi ; Qin, Xiangmou ; Wu, Hui ; Feng, Hao ; Zhang, Yong-An ; Tu, Jiagang</creatorcontrib><description>Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) plays an important role in regulating the replication of many viruses. However, it remains elusive whether and how hnRNPA1 regulates fish virus replication. In this study, the effects of twelve hnRNPs on the replication of snakehead vesiculovirus (SHVV) were screened. Three hnRNPs, one of which was hnRNPA1, were identified as anti-SHVV factors. Further verification showed that knockdown of hnRNPA1 promoted, while overexpression of hnRNPA1 inhibited, SHVV replication. SHVV infection reduced the expression level of hnRNPA1 and induced the nucleocytoplasmic shuttling of hnRNPA1. Besides, we found that hnRNPA1 interacted with the viral phosphoprotein (P) via its glycine-rich domain, but not with the viral nucleoprotein (N) or large protein (L). The hnRNPA1-P interaction competitively disrupted the viral P-N interaction. Moreover, we found that overexpression of hnRNPA1 enhanced the polyubiquitination of the P protein and degraded it through proteasomal and lysosomal pathways. This study will help understanding the function of hnRNPA1 in the replication of single-stranded negative-sense RNA viruses and providing a novel antiviral target against fish rhabdoviruses.</description><identifier>ISSN: 2150-5594</identifier><identifier>EISSN: 2150-5608</identifier><identifier>DOI: 10.1080/21505594.2023.2196847</identifier><identifier>PMID: 37005771</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Animals ; Fishes ; Heterogeneous Nuclear Ribonucleoprotein A1 - genetics ; HnRNPA1 ; Nucleocytoplasmic shuttling ; Nucleoproteins - metabolism ; P protein ; Phosphoproteins - metabolism ; Replication ; Rhabdoviridae Infections - metabolism ; Snakehead vesiculovirus (SHVV) ; Vesiculovirus - genetics ; Vesiculovirus - metabolism ; Virus Replication</subject><ispartof>Virulence, 2023-12, Vol.14 (1), p.2196847-2196847</ispartof><rights>2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2023</rights><rights>2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2023 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c483t-45b6150b1c1bbcf4cc06b792a1bfb6eca794275decaef707ea9b28964009498d3</cites><orcidid>0000-0001-5447-4699</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072109/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072109/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27485,27907,27908,53774,53776,59124,59125</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37005771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, An-Qi</creatorcontrib><creatorcontrib>Qin, Xiangmou</creatorcontrib><creatorcontrib>Wu, Hui</creatorcontrib><creatorcontrib>Feng, Hao</creatorcontrib><creatorcontrib>Zhang, Yong-An</creatorcontrib><creatorcontrib>Tu, Jiagang</creatorcontrib><title>hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein</title><title>Virulence</title><addtitle>Virulence</addtitle><description>Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) plays an important role in regulating the replication of many viruses. However, it remains elusive whether and how hnRNPA1 regulates fish virus replication. In this study, the effects of twelve hnRNPs on the replication of snakehead vesiculovirus (SHVV) were screened. Three hnRNPs, one of which was hnRNPA1, were identified as anti-SHVV factors. Further verification showed that knockdown of hnRNPA1 promoted, while overexpression of hnRNPA1 inhibited, SHVV replication. SHVV infection reduced the expression level of hnRNPA1 and induced the nucleocytoplasmic shuttling of hnRNPA1. Besides, we found that hnRNPA1 interacted with the viral phosphoprotein (P) via its glycine-rich domain, but not with the viral nucleoprotein (N) or large protein (L). The hnRNPA1-P interaction competitively disrupted the viral P-N interaction. Moreover, we found that overexpression of hnRNPA1 enhanced the polyubiquitination of the P protein and degraded it through proteasomal and lysosomal pathways. This study will help understanding the function of hnRNPA1 in the replication of single-stranded negative-sense RNA viruses and providing a novel antiviral target against fish rhabdoviruses.</description><subject>Animals</subject><subject>Fishes</subject><subject>Heterogeneous Nuclear Ribonucleoprotein A1 - genetics</subject><subject>HnRNPA1</subject><subject>Nucleocytoplasmic shuttling</subject><subject>Nucleoproteins - metabolism</subject><subject>P protein</subject><subject>Phosphoproteins - metabolism</subject><subject>Replication</subject><subject>Rhabdoviridae Infections - metabolism</subject><subject>Snakehead vesiculovirus (SHVV)</subject><subject>Vesiculovirus - genetics</subject><subject>Vesiculovirus - metabolism</subject><subject>Virus Replication</subject><issn>2150-5594</issn><issn>2150-5608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>DOA</sourceid><recordid>eNp9UsFu1DAQjRCIVqWfAMqRyy6248TJCaqq0EoVIARna2xPdl0SO9jOov0dvrTe7m5FD2DJ8njmvTce6xXFa0qWlLTkHaM1qeuOLxlh1ZLRrmm5eFac7vKLuiHt82OcQSfFeYx3JC_e0kx7WZxUgpBaCHpa_Fm7b5-_XtDSjhMajGV08BPXCKbcYLR6HvzGhjmWAafBakjWu3JjodQ-E5JNdoPDtjQ2hnlK1q1yMcBQTmsf856CT2jdws16wOOttC5hAP2gBc6UBlcBzL_Ir4oXPQwRzw_nWfHj49X3y-vF7ZdPN5cXtwvN2yoteK2aPLKimiqle641aZToGFDVqwY1iI4zUZscYS-IQOgUa7uGE9LxrjXVWXGz1zUe7uQU7AhhKz1Y-ZDwYSUhJJsHkYIxykyljOKKQ_5vMBXvu6qHxgDBJmu932tNsxrRaHQpD_ZE9GnF2bVc-Y2khAhGSZcV3h4Ugv81Y0xytFHjMIBDP0fJ8jhNW4u6zdB6D9XBxxiwf-xDidz5RR79Ind-kQe_ZN6bvx_5yDq6IwM-7AHW9T6M8NuHwcgE28GHPoDTNsrq_z3uAbSu1rI</recordid><startdate>20231231</startdate><enddate>20231231</enddate><creator>Liu, An-Qi</creator><creator>Qin, Xiangmou</creator><creator>Wu, Hui</creator><creator>Feng, Hao</creator><creator>Zhang, Yong-An</creator><creator>Tu, Jiagang</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5447-4699</orcidid></search><sort><creationdate>20231231</creationdate><title>hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein</title><author>Liu, An-Qi ; Qin, Xiangmou ; Wu, Hui ; Feng, Hao ; Zhang, Yong-An ; Tu, Jiagang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-45b6150b1c1bbcf4cc06b792a1bfb6eca794275decaef707ea9b28964009498d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Fishes</topic><topic>Heterogeneous Nuclear Ribonucleoprotein A1 - genetics</topic><topic>HnRNPA1</topic><topic>Nucleocytoplasmic shuttling</topic><topic>Nucleoproteins - metabolism</topic><topic>P protein</topic><topic>Phosphoproteins - metabolism</topic><topic>Replication</topic><topic>Rhabdoviridae Infections - metabolism</topic><topic>Snakehead vesiculovirus (SHVV)</topic><topic>Vesiculovirus - genetics</topic><topic>Vesiculovirus - metabolism</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, An-Qi</creatorcontrib><creatorcontrib>Qin, Xiangmou</creatorcontrib><creatorcontrib>Wu, Hui</creatorcontrib><creatorcontrib>Feng, Hao</creatorcontrib><creatorcontrib>Zhang, Yong-An</creatorcontrib><creatorcontrib>Tu, Jiagang</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Virulence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, An-Qi</au><au>Qin, Xiangmou</au><au>Wu, Hui</au><au>Feng, Hao</au><au>Zhang, Yong-An</au><au>Tu, Jiagang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein</atitle><jtitle>Virulence</jtitle><addtitle>Virulence</addtitle><date>2023-12-31</date><risdate>2023</risdate><volume>14</volume><issue>1</issue><spage>2196847</spage><epage>2196847</epage><pages>2196847-2196847</pages><issn>2150-5594</issn><eissn>2150-5608</eissn><abstract>Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) plays an important role in regulating the replication of many viruses. However, it remains elusive whether and how hnRNPA1 regulates fish virus replication. In this study, the effects of twelve hnRNPs on the replication of snakehead vesiculovirus (SHVV) were screened. Three hnRNPs, one of which was hnRNPA1, were identified as anti-SHVV factors. Further verification showed that knockdown of hnRNPA1 promoted, while overexpression of hnRNPA1 inhibited, SHVV replication. SHVV infection reduced the expression level of hnRNPA1 and induced the nucleocytoplasmic shuttling of hnRNPA1. Besides, we found that hnRNPA1 interacted with the viral phosphoprotein (P) via its glycine-rich domain, but not with the viral nucleoprotein (N) or large protein (L). The hnRNPA1-P interaction competitively disrupted the viral P-N interaction. Moreover, we found that overexpression of hnRNPA1 enhanced the polyubiquitination of the P protein and degraded it through proteasomal and lysosomal pathways. This study will help understanding the function of hnRNPA1 in the replication of single-stranded negative-sense RNA viruses and providing a novel antiviral target against fish rhabdoviruses.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>37005771</pmid><doi>10.1080/21505594.2023.2196847</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5447-4699</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2150-5594 |
ispartof | Virulence, 2023-12, Vol.14 (1), p.2196847-2196847 |
issn | 2150-5594 2150-5608 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_72212d3bdb4b4a608ad34f93fa6da0e6 |
source | PubMed (Medline); Taylor & Francis Open Access |
subjects | Animals Fishes Heterogeneous Nuclear Ribonucleoprotein A1 - genetics HnRNPA1 Nucleocytoplasmic shuttling Nucleoproteins - metabolism P protein Phosphoproteins - metabolism Replication Rhabdoviridae Infections - metabolism Snakehead vesiculovirus (SHVV) Vesiculovirus - genetics Vesiculovirus - metabolism Virus Replication |
title | hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T10%3A24%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=hnRNPA1%20impedes%20snakehead%20vesiculovirus%20replication%20via%20competitively%20disrupting%20viral%20phosphoprotein-nucleoprotein%20interaction%20and%20degrading%20viral%20phosphoprotein&rft.jtitle=Virulence&rft.au=Liu,%20An-Qi&rft.date=2023-12-31&rft.volume=14&rft.issue=1&rft.spage=2196847&rft.epage=2196847&rft.pages=2196847-2196847&rft.issn=2150-5594&rft.eissn=2150-5608&rft_id=info:doi/10.1080/21505594.2023.2196847&rft_dat=%3Cproquest_doaj_%3E2794685758%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c483t-45b6150b1c1bbcf4cc06b792a1bfb6eca794275decaef707ea9b28964009498d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2794685758&rft_id=info:pmid/37005771&rfr_iscdi=true |