A fluorescence imaging technique suggests that sweat leakage in the epidermis contributes to the pathomechanism of palmoplantar pustulosis

• Published in: Scientific reports 2024-01, Vol.14 (1), p.378-378, Article 378
• Main Authors: Yatsuzuka, Kazuki, Kawakami, Ryosuke, Niko, Yosuke, Tsuda, Teruko, Kameda, Kenji, Kohri, Nobushige, Yoshida, Satoshi, Shiraishi, Ken, Muto, Jun, Mori, Hideki, Fujisawa, Yasuhiro, Imamura, Takeshi, Murakami, Masamoto
• Format: Article
• Language: English
• Subjects: 631/1647/245/2186; 631/1647/245/2221; 631/1647/245/2225; 692/698; 692/699/4033; Animals; Cadherins - metabolism; E-cadherin; Eccrine Glands - metabolism; Epidermis; Epidermis - metabolism; Fluorescent indicators; Humanities and Social Sciences; Interleukin 1; Interleukin-1 - metabolism; Keratinocytes; Lasers; Lesions; Mice; multidisciplinary; Optical Imaging - adverse effects; Physiology; Psoriasis - metabolism; Pustulosis; Science; Science (multidisciplinary); Skin; Skin - metabolism; Skin diseases; Skin lesions; Sweat; Sweat - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-50875-x

Sweat is an essential protection system for the body, but its failure can result in pathologic conditions, including several skin diseases, such as palmoplantar pustulosis (PPP). As reduced intraepidermal E-cadherin expression in skin lesions was confirmed in PPP skin lesions, a role for interleukin (IL)-1-rich sweat in PPP has been proposed, and IL-1 has been implicated in the altered E-cadherin expression observed in both cultured keratinocytes and mice epidermis. For further investigation, live imaging of sweat perspiration on a mouse toe-pad under two-photon excitation microscopy was performed using a novel fluorescent dye cocktail (which we named JSAC). Finally, intraepidermal vesicle formation which is the main cause of PPP pathogenesis was successfully induced using our "LASER-snipe" technique with JSAC. "LASER-snipe" is a type of laser ablation technique that uses two-photon absorption of fluorescent material to destroy a few acrosyringium cells at a pinpoint location in three-dimensional space of living tissue to cause eccrine sweat leakage. These observatory techniques and this mouse model may be useful not only in live imaging for physiological phenomena in vivo such as PPP pathomechanism investigation, but also for the field of functional physiological morphology.