The effect of surface morphology on endothelial and smooth muscle cells growth on blow-spun fibrous scaffolds

• Published in: Journal of biological engineering 2021-12, Vol.15 (1), p.27-27, Article 27
• Main Authors: Łopianiak, Iwona, Wojasiński, Michał, Kuźmińska, Aleksandra, Trzaskowska, Paulina, Butruk-Raszeja, Beata A
• Format: Article
• Language: English
• Subjects: Analysis; Bi-layer vascular graft; Endothelial cells; Endothelium; Growth; One-layer vascular graft; Polymers; Polyurethane; Smooth muscle; Smooth muscle cells; Solution blow spinning
• ISSN: 1754-1611; 1754-1611
• DOI: 10.1186/s13036-021-00278-1

This study aimed to analyze the growth of two types of blood vessel building cells: endothelial cells (ECs) and smooth muscle cells (SMCs) on surfaces with different morphology. Two types of materials, differing in morphology, were produced by the solution blow spinning technique. One-layer materials consisted of one fibrous layer with two fibrous surfaces. Bi-layer materials consisted of one fibrous-solid layer and one fibrous layer, resulting in two different surfaces. Additionally, materials with different average fiber diameters (about 200, 500, and 900 nm) were produced for each group. It has been shown that it is possible to obtain structures with a given morphology by changing the selected process parameters (working distance and polymer solution concentration). Both morphology (solid versus fibrous) and average fiber diameter (submicron fibers versus microfibers) of scaffolds influenced the growth of ECs. However, this effect was only visible after an extended period of culture (6 days). In the case of SMCs, it was proved that the best growth of SMCs is obtained for micron fibers (with an average diameter close to 900 nm) compared to the submicron fibers (with an average diameter below 900 nm).