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Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polariza...
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| Published in: | BMC immunology 2021-03, Vol.22 (1), p.21-21, Article 21 |
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| creator | Mohd Idrus, Fatin Najiah Ahmad, Nurul Shuhadah Hoe, Chee Hock Azlan, Maryam Norfuad, Farisha Alia Yusof, Zurkurnai Wan Isa, Wan Yus Haniff Mohamed Ali, Akbar Ali Yvonne-Tee, Get Bee |
| description | Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polarization into M1 and M2 occurs in tissues in concomitance with the acquisition of functional phenotypes depending on specific microenvironment stimuli. However, whether the macrophage differential polarization and MerTK expression vary in coronary artery disease (CAD) patients remain unknown.
This study aimed to elucidate the polarization of M1 and M2 macrophage from CAD patients as well as to investigate the expression of MerTK in these macrophage phenotypes.
A total of 14 (n) CAD patients were recruited and subsequently grouped into "no apparent CAD", "non-obstructive CAD" and "obstructive CAD" according to the degree of stenosis. Thirty ml of venous blood was withdrawn to obtain monocyte from the patients. The M1 macrophage was generated by treating the monocyte with GMCSF, LPS and IFN-γ while MCSF, IL-4 and IL-13 were employed to differentiate monocyte into M2 macrophage. After 7 days of polarization, analysis of cell surface differentiation markers (CD86
/CD80
for M1 and CD206
/CD200R
for M2) and measurement of MerTK expression were performed using flow cytometry.
Both M1 and M2 macrophage expressed similar level of CD86, CD80 and CD206 in all groups of CAD patients. MerTK expression in no apparent CAD patients was significantly higher in M2 macrophage compared to M1 macrophage [12.58 ± 4.40 vs. 6.58 ± 1.37, p = 0.040].
Differential polarization of macrophage into M1 and M2 was highly dynamic and can be varied due to the microenvironment stimuli in atherosclerotic plaque. Besides, higher expression of MerTK in patients with the least coronary obstructive suggest its vital involvement in efferocytosis. |
| doi_str_mv | 10.1186/s12865-021-00410-2 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_72a0752ae2ed440b978a3adb77d5b205</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A656411562</galeid><doaj_id>oai_doaj_org_article_72a0752ae2ed440b978a3adb77d5b205</doaj_id><sourcerecordid>A656411562</sourcerecordid><originalsourceid>FETCH-LOGICAL-c597t-91012e341ba1c2f320f2e65d005ae10b3bf6d09e826737caafc0e28e23bbd1023</originalsourceid><addsrcrecordid>eNptkstu1DAUhiMEoqXwAiyQJTawSPEljpMNUlVuIzpCgrK2TuyTGY-SOLUzqOU5eGCcmVI6CHlh6_g7_7noz7LnjJ4yVpVvIuNVKXPKWU5pwWjOH2THrFAs50zxh_feR9mTGDeUMlXx6nF2JIQqWVXJ4-zXO9e2GHCYHHRk9B0E9xMm5wcCgyXTGglejwFjnEO-JTjj3txMPrpIAhocJx_IEsPlZ5KQJdslLjnpwQQ_rmGFkbiYlCe0pA2-J8YHP0C4IRAmTJd1ESEiGVPh1El8mj1qoYv47PY-yb5_eH95_im_-PJxcX52kRtZqymvGWUcRcEaYIa3gtOWYyktpRKQ0UY0bWlpjRUvlVAGoDUUeYVcNI1llIuTbLHXtR42egyuT01pD07vAj6sdOrQmQ614kCV5IAcbVHQplYVCLCNUlY2nMqk9XavNW6bHq1JcwToDkQPfwa31iv_Q6u65rUQSeDVrUDwV1uMk-5dNNh1MKDfRs1lqlJKVs_oy3_Qjd-GIa0qUbKiaWBe_qVWkAZwQ-tTXTOL6rNSlgVjspx3cPofKh2LvTN-wNal-EHC64OExEx4Pa1gG6NefPt6yPI9m5wQY8D2bh-M6tnDeu9hnTysdx7Wc9KL-5u8S_ljWvEbqortAA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2558082626</pqid></control><display><type>article</type><title>Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Mohd Idrus, Fatin Najiah ; Ahmad, Nurul Shuhadah ; Hoe, Chee Hock ; Azlan, Maryam ; Norfuad, Farisha Alia ; Yusof, Zurkurnai ; Wan Isa, Wan Yus Haniff ; Mohamed Ali, Akbar Ali ; Yvonne-Tee, Get Bee</creator><creatorcontrib>Mohd Idrus, Fatin Najiah ; Ahmad, Nurul Shuhadah ; Hoe, Chee Hock ; Azlan, Maryam ; Norfuad, Farisha Alia ; Yusof, Zurkurnai ; Wan Isa, Wan Yus Haniff ; Mohamed Ali, Akbar Ali ; Yvonne-Tee, Get Bee</creatorcontrib><description>Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polarization into M1 and M2 occurs in tissues in concomitance with the acquisition of functional phenotypes depending on specific microenvironment stimuli. However, whether the macrophage differential polarization and MerTK expression vary in coronary artery disease (CAD) patients remain unknown.
This study aimed to elucidate the polarization of M1 and M2 macrophage from CAD patients as well as to investigate the expression of MerTK in these macrophage phenotypes.
A total of 14 (n) CAD patients were recruited and subsequently grouped into "no apparent CAD", "non-obstructive CAD" and "obstructive CAD" according to the degree of stenosis. Thirty ml of venous blood was withdrawn to obtain monocyte from the patients. The M1 macrophage was generated by treating the monocyte with GMCSF, LPS and IFN-γ while MCSF, IL-4 and IL-13 were employed to differentiate monocyte into M2 macrophage. After 7 days of polarization, analysis of cell surface differentiation markers (CD86
/CD80
for M1 and CD206
/CD200R
for M2) and measurement of MerTK expression were performed using flow cytometry.
Both M1 and M2 macrophage expressed similar level of CD86, CD80 and CD206 in all groups of CAD patients. MerTK expression in no apparent CAD patients was significantly higher in M2 macrophage compared to M1 macrophage [12.58 ± 4.40 vs. 6.58 ± 1.37, p = 0.040].
Differential polarization of macrophage into M1 and M2 was highly dynamic and can be varied due to the microenvironment stimuli in atherosclerotic plaque. Besides, higher expression of MerTK in patients with the least coronary obstructive suggest its vital involvement in efferocytosis.</description><identifier>ISSN: 1471-2172</identifier><identifier>EISSN: 1471-2172</identifier><identifier>DOI: 10.1186/s12865-021-00410-2</identifier><identifier>PMID: 33761885</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Arteriosclerosis ; Atherosclerosis ; c-Mer Tyrosine Kinase - metabolism ; Cardiovascular disease ; Carotid arteries ; CD80 antigen ; CD86 antigen ; Cell Differentiation ; Cell surface ; Cell surface differentiation marker ; Cellular Microenvironment ; Coronary artery ; Coronary artery disease ; Coronary Artery Disease - immunology ; Coronary heart disease ; Coronary vessels ; Coronary Vessels - pathology ; Cytokines ; Cytokines - metabolism ; Development and progression ; Efferocytosis ; Endocytosis ; Female ; Flow Cytometry ; Genetic aspects ; Health aspects ; Heart diseases ; Humans ; Interleukin 13 ; Interleukin 4 ; Macrophage polarization ; Macrophages ; Macrophages - immunology ; Male ; Mer proto-oncogene tyrosine kinase ; Microenvironments ; Middle Aged ; Monocytes ; Pathogens ; Patients ; Phagocytosis ; Phenotypes ; Polarization ; Protein tyrosine kinase ; Stenosis ; Th1 Cells - immunology ; Th2 Cells - immunology ; Tumor necrosis factor-TNF ; Up-Regulation ; γ-Interferon</subject><ispartof>BMC immunology, 2021-03, Vol.22 (1), p.21-21, Article 21</ispartof><rights>COPYRIGHT 2021 BioMed Central Ltd.</rights><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c597t-91012e341ba1c2f320f2e65d005ae10b3bf6d09e826737caafc0e28e23bbd1023</citedby><cites>FETCH-LOGICAL-c597t-91012e341ba1c2f320f2e65d005ae10b3bf6d09e826737caafc0e28e23bbd1023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992933/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2558082626?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33761885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohd Idrus, Fatin Najiah</creatorcontrib><creatorcontrib>Ahmad, Nurul Shuhadah</creatorcontrib><creatorcontrib>Hoe, Chee Hock</creatorcontrib><creatorcontrib>Azlan, Maryam</creatorcontrib><creatorcontrib>Norfuad, Farisha Alia</creatorcontrib><creatorcontrib>Yusof, Zurkurnai</creatorcontrib><creatorcontrib>Wan Isa, Wan Yus Haniff</creatorcontrib><creatorcontrib>Mohamed Ali, Akbar Ali</creatorcontrib><creatorcontrib>Yvonne-Tee, Get Bee</creatorcontrib><title>Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients</title><title>BMC immunology</title><addtitle>BMC Immunol</addtitle><description>Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polarization into M1 and M2 occurs in tissues in concomitance with the acquisition of functional phenotypes depending on specific microenvironment stimuli. However, whether the macrophage differential polarization and MerTK expression vary in coronary artery disease (CAD) patients remain unknown.
This study aimed to elucidate the polarization of M1 and M2 macrophage from CAD patients as well as to investigate the expression of MerTK in these macrophage phenotypes.
A total of 14 (n) CAD patients were recruited and subsequently grouped into "no apparent CAD", "non-obstructive CAD" and "obstructive CAD" according to the degree of stenosis. Thirty ml of venous blood was withdrawn to obtain monocyte from the patients. The M1 macrophage was generated by treating the monocyte with GMCSF, LPS and IFN-γ while MCSF, IL-4 and IL-13 were employed to differentiate monocyte into M2 macrophage. After 7 days of polarization, analysis of cell surface differentiation markers (CD86
/CD80
for M1 and CD206
/CD200R
for M2) and measurement of MerTK expression were performed using flow cytometry.
Both M1 and M2 macrophage expressed similar level of CD86, CD80 and CD206 in all groups of CAD patients. MerTK expression in no apparent CAD patients was significantly higher in M2 macrophage compared to M1 macrophage [12.58 ± 4.40 vs. 6.58 ± 1.37, p = 0.040].
Differential polarization of macrophage into M1 and M2 was highly dynamic and can be varied due to the microenvironment stimuli in atherosclerotic plaque. Besides, higher expression of MerTK in patients with the least coronary obstructive suggest its vital involvement in efferocytosis.</description><subject>Adult</subject><subject>Arteriosclerosis</subject><subject>Atherosclerosis</subject><subject>c-Mer Tyrosine Kinase - metabolism</subject><subject>Cardiovascular disease</subject><subject>Carotid arteries</subject><subject>CD80 antigen</subject><subject>CD86 antigen</subject><subject>Cell Differentiation</subject><subject>Cell surface</subject><subject>Cell surface differentiation marker</subject><subject>Cellular Microenvironment</subject><subject>Coronary artery</subject><subject>Coronary artery disease</subject><subject>Coronary Artery Disease - immunology</subject><subject>Coronary heart disease</subject><subject>Coronary vessels</subject><subject>Coronary Vessels - pathology</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Development and progression</subject><subject>Efferocytosis</subject><subject>Endocytosis</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Heart diseases</subject><subject>Humans</subject><subject>Interleukin 13</subject><subject>Interleukin 4</subject><subject>Macrophage polarization</subject><subject>Macrophages</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Mer proto-oncogene tyrosine kinase</subject><subject>Microenvironments</subject><subject>Middle Aged</subject><subject>Monocytes</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Phagocytosis</subject><subject>Phenotypes</subject><subject>Polarization</subject><subject>Protein tyrosine kinase</subject><subject>Stenosis</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><subject>Tumor necrosis factor-TNF</subject><subject>Up-Regulation</subject><subject>γ-Interferon</subject><issn>1471-2172</issn><issn>1471-2172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkstu1DAUhiMEoqXwAiyQJTawSPEljpMNUlVuIzpCgrK2TuyTGY-SOLUzqOU5eGCcmVI6CHlh6_g7_7noz7LnjJ4yVpVvIuNVKXPKWU5pwWjOH2THrFAs50zxh_feR9mTGDeUMlXx6nF2JIQqWVXJ4-zXO9e2GHCYHHRk9B0E9xMm5wcCgyXTGglejwFjnEO-JTjj3txMPrpIAhocJx_IEsPlZ5KQJdslLjnpwQQ_rmGFkbiYlCe0pA2-J8YHP0C4IRAmTJd1ESEiGVPh1El8mj1qoYv47PY-yb5_eH95_im_-PJxcX52kRtZqymvGWUcRcEaYIa3gtOWYyktpRKQ0UY0bWlpjRUvlVAGoDUUeYVcNI1llIuTbLHXtR42egyuT01pD07vAj6sdOrQmQ614kCV5IAcbVHQplYVCLCNUlY2nMqk9XavNW6bHq1JcwToDkQPfwa31iv_Q6u65rUQSeDVrUDwV1uMk-5dNNh1MKDfRs1lqlJKVs_oy3_Qjd-GIa0qUbKiaWBe_qVWkAZwQ-tTXTOL6rNSlgVjspx3cPofKh2LvTN-wNal-EHC64OExEx4Pa1gG6NefPt6yPI9m5wQY8D2bh-M6tnDeu9hnTysdx7Wc9KL-5u8S_ljWvEbqortAA</recordid><startdate>20210324</startdate><enddate>20210324</enddate><creator>Mohd Idrus, Fatin Najiah</creator><creator>Ahmad, Nurul Shuhadah</creator><creator>Hoe, Chee Hock</creator><creator>Azlan, Maryam</creator><creator>Norfuad, Farisha Alia</creator><creator>Yusof, Zurkurnai</creator><creator>Wan Isa, Wan Yus Haniff</creator><creator>Mohamed Ali, Akbar Ali</creator><creator>Yvonne-Tee, Get Bee</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210324</creationdate><title>Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients</title><author>Mohd Idrus, Fatin Najiah ; Ahmad, Nurul Shuhadah ; Hoe, Chee Hock ; Azlan, Maryam ; Norfuad, Farisha Alia ; Yusof, Zurkurnai ; Wan Isa, Wan Yus Haniff ; Mohamed Ali, Akbar Ali ; Yvonne-Tee, Get Bee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c597t-91012e341ba1c2f320f2e65d005ae10b3bf6d09e826737caafc0e28e23bbd1023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Arteriosclerosis</topic><topic>Atherosclerosis</topic><topic>c-Mer Tyrosine Kinase - metabolism</topic><topic>Cardiovascular disease</topic><topic>Carotid arteries</topic><topic>CD80 antigen</topic><topic>CD86 antigen</topic><topic>Cell Differentiation</topic><topic>Cell surface</topic><topic>Cell surface differentiation marker</topic><topic>Cellular Microenvironment</topic><topic>Coronary artery</topic><topic>Coronary artery disease</topic><topic>Coronary Artery Disease - immunology</topic><topic>Coronary heart disease</topic><topic>Coronary vessels</topic><topic>Coronary Vessels - pathology</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Development and progression</topic><topic>Efferocytosis</topic><topic>Endocytosis</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Heart diseases</topic><topic>Humans</topic><topic>Interleukin 13</topic><topic>Interleukin 4</topic><topic>Macrophage polarization</topic><topic>Macrophages</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Mer proto-oncogene tyrosine kinase</topic><topic>Microenvironments</topic><topic>Middle Aged</topic><topic>Monocytes</topic><topic>Pathogens</topic><topic>Patients</topic><topic>Phagocytosis</topic><topic>Phenotypes</topic><topic>Polarization</topic><topic>Protein tyrosine kinase</topic><topic>Stenosis</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - immunology</topic><topic>Tumor necrosis factor-TNF</topic><topic>Up-Regulation</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohd Idrus, Fatin Najiah</creatorcontrib><creatorcontrib>Ahmad, Nurul Shuhadah</creatorcontrib><creatorcontrib>Hoe, Chee Hock</creatorcontrib><creatorcontrib>Azlan, Maryam</creatorcontrib><creatorcontrib>Norfuad, Farisha Alia</creatorcontrib><creatorcontrib>Yusof, Zurkurnai</creatorcontrib><creatorcontrib>Wan Isa, Wan Yus Haniff</creatorcontrib><creatorcontrib>Mohamed Ali, Akbar Ali</creatorcontrib><creatorcontrib>Yvonne-Tee, Get Bee</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>BMC immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohd Idrus, Fatin Najiah</au><au>Ahmad, Nurul Shuhadah</au><au>Hoe, Chee Hock</au><au>Azlan, Maryam</au><au>Norfuad, Farisha Alia</au><au>Yusof, Zurkurnai</au><au>Wan Isa, Wan Yus Haniff</au><au>Mohamed Ali, Akbar Ali</au><au>Yvonne-Tee, Get Bee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients</atitle><jtitle>BMC immunology</jtitle><addtitle>BMC Immunol</addtitle><date>2021-03-24</date><risdate>2021</risdate><volume>22</volume><issue>1</issue><spage>21</spage><epage>21</epage><pages>21-21</pages><artnum>21</artnum><issn>1471-2172</issn><eissn>1471-2172</eissn><abstract>Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polarization into M1 and M2 occurs in tissues in concomitance with the acquisition of functional phenotypes depending on specific microenvironment stimuli. However, whether the macrophage differential polarization and MerTK expression vary in coronary artery disease (CAD) patients remain unknown.
This study aimed to elucidate the polarization of M1 and M2 macrophage from CAD patients as well as to investigate the expression of MerTK in these macrophage phenotypes.
A total of 14 (n) CAD patients were recruited and subsequently grouped into "no apparent CAD", "non-obstructive CAD" and "obstructive CAD" according to the degree of stenosis. Thirty ml of venous blood was withdrawn to obtain monocyte from the patients. The M1 macrophage was generated by treating the monocyte with GMCSF, LPS and IFN-γ while MCSF, IL-4 and IL-13 were employed to differentiate monocyte into M2 macrophage. After 7 days of polarization, analysis of cell surface differentiation markers (CD86
/CD80
for M1 and CD206
/CD200R
for M2) and measurement of MerTK expression were performed using flow cytometry.
Both M1 and M2 macrophage expressed similar level of CD86, CD80 and CD206 in all groups of CAD patients. MerTK expression in no apparent CAD patients was significantly higher in M2 macrophage compared to M1 macrophage [12.58 ± 4.40 vs. 6.58 ± 1.37, p = 0.040].
Differential polarization of macrophage into M1 and M2 was highly dynamic and can be varied due to the microenvironment stimuli in atherosclerotic plaque. Besides, higher expression of MerTK in patients with the least coronary obstructive suggest its vital involvement in efferocytosis.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>33761885</pmid><doi>10.1186/s12865-021-00410-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1471-2172 |
| ispartof | BMC immunology, 2021-03, Vol.22 (1), p.21-21, Article 21 |
| issn | 1471-2172 1471-2172 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_72a0752ae2ed440b978a3adb77d5b205 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Adult Arteriosclerosis Atherosclerosis c-Mer Tyrosine Kinase - metabolism Cardiovascular disease Carotid arteries CD80 antigen CD86 antigen Cell Differentiation Cell surface Cell surface differentiation marker Cellular Microenvironment Coronary artery Coronary artery disease Coronary Artery Disease - immunology Coronary heart disease Coronary vessels Coronary Vessels - pathology Cytokines Cytokines - metabolism Development and progression Efferocytosis Endocytosis Female Flow Cytometry Genetic aspects Health aspects Heart diseases Humans Interleukin 13 Interleukin 4 Macrophage polarization Macrophages Macrophages - immunology Male Mer proto-oncogene tyrosine kinase Microenvironments Middle Aged Monocytes Pathogens Patients Phagocytosis Phenotypes Polarization Protein tyrosine kinase Stenosis Th1 Cells - immunology Th2 Cells - immunology Tumor necrosis factor-TNF Up-Regulation γ-Interferon |
| title | Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T14%3A02%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20polarization%20and%20the%20expression%20of%20efferocytosis%20receptor%20MerTK%20on%20M1%20and%20M2%20macrophages%20isolated%20from%20coronary%20artery%20disease%20patients&rft.jtitle=BMC%20immunology&rft.au=Mohd%20Idrus,%20Fatin%20Najiah&rft.date=2021-03-24&rft.volume=22&rft.issue=1&rft.spage=21&rft.epage=21&rft.pages=21-21&rft.artnum=21&rft.issn=1471-2172&rft.eissn=1471-2172&rft_id=info:doi/10.1186/s12865-021-00410-2&rft_dat=%3Cgale_doaj_%3EA656411562%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c597t-91012e341ba1c2f320f2e65d005ae10b3bf6d09e826737caafc0e28e23bbd1023%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2558082626&rft_id=info:pmid/33761885&rft_galeid=A656411562&rfr_iscdi=true |