Investigation of Pomegranate ( Punica granatum L.) Flowers' Antioxidant Properties and Antibacterial Activities against Different Staphylococcus Species Associated with Bovine Mastitis

Mastitis is one of the most considerable and costly diseases for dairy herds, and spp. is known to be the main causative agent. Although antibiotics are widely used in the treatment of mastitis, this can cause both antibiotic residues in milk and the risk of antibiotic resistance occurrence in bacte...

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Published in:Veterinary sciences 2023-06, Vol.10 (6), p.394
Main Authors: Ökmen, Gülten, Giannetto, Daniela, Fazio, Francesco, Arslan, Kutbettin
Format: Article
Language:English
Subjects:
Animals
Antibacterial activity
Antibiotic resistance
Antibiotics
Antimicrobial agents
antioxidant activity
Antioxidants
Bacteria
Bacterial infections
Chromatography
Coagulase
Dairy industry
Ethanol
Flavonoids
Flowers
Flowers & plants
Free radicals
Fruits
Laboratories
Mastitis
Medicinal plants
Metabolites
Methanol
Natural products
Ornamental plants
Oxidative stress
Pathogens
Polyphenols
Public health
Punica
Punica granatum
Seeds
Solvents
Staphylococcus
Thin-layer chromatography
TLC
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description Mastitis is one of the most considerable and costly diseases for dairy herds, and spp. is known to be the main causative agent. Although antibiotics are widely used in the treatment of mastitis, this can cause both antibiotic residues in milk and the risk of antibiotic resistance occurrence in bacteria. Thus, in recent years, researchers have focused on alternative treatments for this disease and plants extracts are investigated for this purpose. Pomegranate is widely used as a dye, ornament, and medicinal plants in the industry, and the species has a particularly high economic value in Turkey. This study aims to investigate in vitro the antioxidant and antibacterial properties of the pomegranate flower's extracts against different species associated with bovine mastitis. To this aim, pomegranate flowers were collected from different regions in Turkey and extracts were prepared with three different solvents (methanol, ethanol, and water). The retention factor values of the ethanol extract were determined by thin-layer chromatography. The antibacterial activity tests were carried out via the disk diffusion method. In addition, the extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radicals for antioxidant activity. Four retention factors (0.79, 0.67, 0.58, and 0.33 points) were found for the ethanol extract. The methanol extract showed the highest inhibition zones against coagulase-negative -37 (CNS-37) and -18. The lowest MIC was 6500 μg/mL. The highest antioxidant activity was observed in methanol extracts. As a result, the extracts of pomegranate flowers showed a high antioxidant and antibacterial potential against the examined mastitis pathogens.
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Flowers' Antioxidant Properties and Antibacterial Activities against Different Staphylococcus Species Associated with Bovine Mastitis</atitle><jtitle>Veterinary sciences</jtitle><addtitle>Vet Sci</addtitle><date>2023-06-11</date><risdate>2023</risdate><volume>10</volume><issue>6</issue><spage>394</spage><pages>394-</pages><issn>2306-7381</issn><eissn>2306-7381</eissn><abstract>Mastitis is one of the most considerable and costly diseases for dairy herds, and spp. is known to be the main causative agent. Although antibiotics are widely used in the treatment of mastitis, this can cause both antibiotic residues in milk and the risk of antibiotic resistance occurrence in bacteria. Thus, in recent years, researchers have focused on alternative treatments for this disease and plants extracts are investigated for this purpose. Pomegranate is widely used as a dye, ornament, and medicinal plants in the industry, and the species has a particularly high economic value in Turkey. This study aims to investigate in vitro the antioxidant and antibacterial properties of the pomegranate flower's extracts against different species associated with bovine mastitis. To this aim, pomegranate flowers were collected from different regions in Turkey and extracts were prepared with three different solvents (methanol, ethanol, and water). The retention factor values of the ethanol extract were determined by thin-layer chromatography. The antibacterial activity tests were carried out via the disk diffusion method. In addition, the extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radicals for antioxidant activity. Four retention factors (0.79, 0.67, 0.58, and 0.33 points) were found for the ethanol extract. The methanol extract showed the highest inhibition zones against coagulase-negative -37 (CNS-37) and -18. The lowest MIC was 6500 μg/mL. The highest antioxidant activity was observed in methanol extracts. 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source Publicly Available Content Database; PubMed Central
subjects Animals
Antibacterial activity
Antibiotic resistance
Antibiotics
Antimicrobial agents
antioxidant activity
Antioxidants
Bacteria
Bacterial infections
Chromatography
Coagulase
Dairy industry
Ethanol
Flavonoids
Flowers
Flowers & plants
Free radicals
Fruits
Laboratories
Mastitis
Medicinal plants
Metabolites
Methanol
Natural products
Ornamental plants
Oxidative stress
Pathogens
Polyphenols
Public health
Punica
Punica granatum
Seeds
Solvents
Staphylococcus
Thin-layer chromatography
TLC
title Investigation of Pomegranate ( Punica granatum L.) Flowers' Antioxidant Properties and Antibacterial Activities against Different Staphylococcus Species Associated with Bovine Mastitis
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