Interaction between PAF and human platelet membranes: a fluorescence study

THE interaction between PAF and human platelet membranes was investigated by measuring the steady-state fluorescence anisotropy and fluorescence decay of 1- (4-trimethylammoniumphenyl) -6-phenyl-1,3,5 -hexatriene (TMA-DPH) incorporated in platelet plasma membranes. PAF induced a time-limited and sig...

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Bibliographic Details
Published in:Mediators of Inflammation 1992, Vol.1992 (2), p.127-131
Main Authors: Kantar, A, Giorgi, P L, Curatola, G, Fiorini, R
Format: Article
Language:English
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Summary:THE interaction between PAF and human platelet membranes was investigated by measuring the steady-state fluorescence anisotropy and fluorescence decay of 1- (4-trimethylammoniumphenyl) -6-phenyl-1,3,5 -hexatriene (TMA-DPH) incorporated in platelet plasma membranes. PAF induced a time-limited and significant increase of the lipid order in the exterior part of the membrane and a decrease in membrane heterogeneity. These changes were blocked in the presence of the PAF antagonists, L-659,989 and 1-O-hexadecyl-2-acetyl-snglycero- 3-phospho(N,N,N-trimethyl)hexanolamine.H_2O. These results indicate that the observed changes in the physico-chemical properties of the membrane are attributed to the PAF-receptor interaction and signal transduction.
ISSN:0962-9351
1466-1861
DOI:10.1155/S0962935192000218