Polysaccharide extract of Mimosa tenuiflora stem barks stimulates acute inflammatory response via nitric oxide

Mimosa tenuiflora (Mimosaceae) or jurema-preta is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammat...

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Bibliographic Details
Published in:Acta scientiarum. Biological sciences 2016, Vol.38 (4), p.473
Main Authors: Silva-Leite, Kaira Emanuella Sales da, Queiroz, Cinthia Silva, Madeira, Juliana Da Costa, Soares, Pedro Marcos Gomes, Pereira, Maria Gonçalves, Assreuy, Ana Maria Sampaio
Format: Article
Language:English
Subjects:
carbohydrate
Carbohydrates
Cell adhesion & migration
Herbal medicine
Inflammation
Leukocytes
medicinal plant
Mimosaceae
Nitric oxide
Trees
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Summary:Mimosa tenuiflora (Mimosaceae) or jurema-preta is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt) by methanol/NaOH and ethanol precipitation. Inflammatory activity was assessed in rat models of acute inflammation (paw edema and peritonitis), by the following parameters: edema, vascular permeability, leukocyte migration, myeloperoxidase activity and pharmacological modulation of nitric oxide and prostaglandins. EP-Mt presented 3.8% yield, 41% carbohydrate and 0.34% protein. EP-Mt (0.01, 0.1, 1.0 mg kg-1) injected by subcutaneous route elicited paw edema that lasted from 30-420 min, with maximal effect at 1 mg kg-1 (40x vs. saline), and was inhibited by L-NAME (52%) and dexamethasone (26%). EP-Mt (1 mg kg-1, via intraperitoneal) stimulated leukocytes migration (2.2x), mainly neutrophils (6.5x) and MPO activity (96%). The leukocyte migration elicited by EP-Mt was inhibited by dexamethasone (39%) and L-NAME (38%). EP-Mt containing high carbohydrate content induces acute inflammation via nitric oxide, which open perspectives of application in pathological conditions of immunosuppression.
ISSN:1679-9283
1807-863X
DOI:10.4025/actascibiolsci.v38i4.32234