The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene

Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-01, Vol.24 (2), p.1798
Main Authors: Li, Charng-Pei, Wu, Dong-Hong, Huang, Shou-Horng, Meng, Menghsiao, Shih, Hsien-Tzung, Lai, Ming-Hsin, Chen, Liang-Jwu, Jena, Kshirod K, Hechanova, Sherry Lou, Ke, Ting-Jyun, Chiu, Tai-Yuan, Tsai, Zong-Yuan, Chen, Guo-Kai, Tsai, Kuan-Chieh, Leu, Wei-Ming
Format: Article
Language:English
Subjects:
Animals
antixenosis
brown planthopper (BPH)
Chromosome 4
Chromosomes
Gene loci
Genes, Plant
Genomes
Hemiptera - genetics
Humans
Limonene
Male
Nilaparvata lugens
Oryza - genetics
Oryza nivara
Oryza sativa japonica
Pest resistance
Plant Diseases - genetics
Prostatic Hyperplasia - genetics
Tainan 11 (TN11)
Tainung 71 (TNG71)
Virulence
VOCs
Volatile organic compounds
Wilting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24021798