The Bph45 Gene Confers Resistance against Brown Planthopper in Rice by Reducing the Production of Limonene

• Published in: International journal of molecular sciences 2023-01, Vol.24 (2), p.1798
• Main Authors: Li, Charng-Pei, Wu, Dong-Hong, Huang, Shou-Horng, Meng, Menghsiao, Shih, Hsien-Tzung, Lai, Ming-Hsin, Chen, Liang-Jwu, Jena, Kshirod K, Hechanova, Sherry Lou, Ke, Ting-Jyun, Chiu, Tai-Yuan, Tsai, Zong-Yuan, Chen, Guo-Kai, Tsai, Kuan-Chieh, Leu, Wei-Ming
• Format: Article
• Language: English
• Subjects: Animals; antixenosis; brown planthopper (BPH); Chromosome 4; Chromosomes; Gene loci; Genes, Plant; Genomes; Hemiptera - genetics; Humans; Limonene; Male; Nilaparvata lugens; Oryza - genetics; Oryza nivara; Oryza sativa japonica; Pest resistance; Plant Diseases - genetics; Prostatic Hyperplasia - genetics; Tainan 11 (TN11); Tainung 71 (TNG71); Virulence; VOCs; Volatile organic compounds; Wilting
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24021798

Brown planthopper (BPH), a monophagous phloem feeder, consumes a large amount of photoassimilates in rice and causes wilting. A near-isogenic line ‘TNG71-Bph45’ was developed from the Oryza sativa japonica variety ‘Tainung 71 (TNG71) carrying a dominant BPH-resistance locus derived from Oryza nivara (IRGC 102165) near the centromere of chromosome 4. We compared the NIL (TNG71-Bph45) and the recurrent parent to explore how the Bph45 gene confers BPH resistance. We found that TNG71-Bph45 is less attractive to BPH at least partially because it produces less limonene. Chiral analysis revealed that the major form of limonene in both rice lines was the L-form. However, both L- and D-limonene attracted BPH when applied exogenously to TNG71-Bph45 rice. The transcript amounts of limonene synthase were significantly higher in TNG71 than in TNG71-Bph45 and were induced by BPH infestation only in the former. Introgression of the Bph45 gene into another japonica variety, Tainan 11, also resulted in a low limonene content. Moreover, several dominantly acting BPH resistance genes introduced into the BPH-sensitive IR24 line compromised its limonene-producing ability and concurrently decreased its attractiveness to BPH. These observations suggest that reducing limonene production may be a common resistance strategy against BPH in rice.