Two New Species of Filamentous Sulfur Bacteria of the Genus Thiothrix, Thiothrix winogradskyi sp. nov. and ‘Candidatus Thiothrix sulfatifontis’ sp. nov

The metagenome of foulings from sulfidic spring “Serovodorodny” (Tatarstan, Russia), where members of the genus Thiothrix was observed, was sequenced. Representatives of the phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in the microbial community. The complete genome of Th...

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Bibliographic Details
Published in:Microorganisms (Basel) 2022-06, Vol.10 (7), p.1300
Main Authors: Ravin, Nikolai, Rossetti, Simona, Beletsky, Alexey, Kadnikov, Vitaly, Rudenko, Tatyana, Smolyakov, Dmitry, Moskvitina, Marina, Gureeva, Maria, Mardanov, Andrey, Grabovich, Margarita
Format: Article
Language:English
Subjects:
ANI
Bacteria
Cyanobacteria
dDDH
Deoxyribonucleic acid
DNA
DNA sequencing
genome
Genomes
Hybridization
Hydrogen sulfide
MAG
Microorganisms
New species
Nucleotide sequence
Nucleotides
Phylogenetics
rRNA 16S
Sulfur
Sulfur bacteria
Taxonomy
Thiosulfate
Thiosulfates
Thiothrix
Water treatment
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Summary:The metagenome of foulings from sulfidic spring “Serovodorodny” (Tatarstan, Russia), where members of the genus Thiothrix was observed, was sequenced. Representatives of the phyla Gammaproteobacteria, Cyanobacteria and Campilobacteriota dominated in the microbial community. The complete genome of Thiothrix sp. KT was assembled from the metagenome. It displayed 93.93–99.72% 16S rRNA gene sequence identity to other Thiothrix species. The average nucleotide identity (ANI) и digital DNA-DNA hybridization (dDDH) showed that the genome designated KT represents a new species within the genus Thiothrix, ‘Candidatus Thiothrix sulfatifontis’ sp. nov. KT. The taxonomic status has been determined of the strain Thiothrix sp. CT3, isolated about 30 years ago and not assigned to any of Thiothrix species due to high 16S rRNA gene sequence identity with related species (i.e., 98.8–99.4%). The complete genome sequence of strain CT3 was determined. The ANI between CT3 and other Thiothrix species was below 82%, and the dDDH values were less than 40%, indicating that strain CT3 belongs to a novel species, Thiothrix winogradskyi sp. nov. A genome analysis showed that both strains are chemo-organoheterotrophs, chemolithotrophs (in the presence of hydrogen sulfide and thiosulfate) and chemoautotrophs. For the first time, representatives of Thiothrix showed anaerobic growth in the presence of thiosulfate.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10071300