A brain microdialysis study on 5-HT release in freely moving rat lines selectively bred for differential 5-HT1A receptor function

• Published in: Brazilian journal of medical and biological research 2003-02, Vol.36 (2), p.263-267
• Main Authors: Gonzalez, L E, Parada, M A, Tucci, S, Teneud, L, Overstreet, D H, Hernandez, L
• Format: Article
• Language: English
• Subjects: 5-HT1A rat lines; 8-Hydroxy-2-(di-n-propylamino)tetralin - pharmacology; 8-OH-DPAT; Analysis of Variance; Animals; BIOLOGY; Brain - drug effects; Brain - metabolism; Brain microdialysis; Breeding; Cerebral Cortex - drug effects; Cerebral Cortex - metabolism; Fenfluramine; Fenfluramine - pharmacology; Hippocampus - drug effects; Hippocampus - metabolism; Hypothermia - metabolism; MEDICINE, RESEARCH & EXPERIMENTAL; Microdialysis; Rats; Receptors, Serotonin - metabolism; Receptors, Serotonin, 5-HT1; Serotonin; Serotonin - metabolism; Serotonin Receptor Agonists - pharmacology; Serotonin Uptake Inhibitors - pharmacology; Species Specificity
• ISSN: 0100-879X; 1414-431X; 0100-879X; 1414-431X
• DOI: 10.1590/S0100-879X2003000200014

Breeding for high and low hypothermic responses to systemic administration of a serotonin1A (5-HT1A) receptor agonist (8-hydroxy-2-(di-n-propylamino)tetralin, 8-OH-DPAT) has resulted in high DPAT-sensitive (HDS) and low DPAT-sensitive (LDS) lines of rats, respectively. These lines also differ in several behavioral measures associated with stress. In the present microdialysis study we observed that basal 5-HT concentrations in the prefrontal cortex and dorsal hippocampus did not differ significantly between HDS and LDS rats. Thus, behavioral differences between the HDS and LDS lines might not be attributed to differences in basal 5-HT release. However, both lines had lower basal levels of 5-HT release than their randomly bred control group (random DPAT-sensitive, RDS) in the prefrontal cortex (mean +/- SEM, pg/20 microl, was 3.0 +/- 0.4 for LDS, 3.8 +/- 0.3 for HDS and 6.4 +/- 0.6 for RDS; F(2,59) = 5.8, P