Virulence Characteristics and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Escherichia coli Isolates from Humans in South Africa: 2006-2013

Shiga toxin-producing (STEC) isolates (N = 38) that were incriminated in human disease from 2006 to 2013 in South Africa were characterized by serotype, virulence-associated genes, antimicrobial resistance and pulsed-field gel electrophoresis (PFGE). The isolates belonged to 11 O:H serotypes. STEC O...

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Bibliographic Details
Published in:Toxins 2019-07, Vol.11 (7), p.424
Main Authors: Karama, Musafiri, Cenci-Goga, Beniamino T, Malahlela, Mogaugedi, Smith, Anthony M, Keddy, Karen H, El-Ashram, Saeed, Kabiru, Lawan M, Kalake, Alan
Format: Article
Language:English
Subjects:
Animals
Antimicrobial agents
Antimicrobial resistance
Disease control
Drug resistance
E coli
Electrophoresis
Epidemics
Epidemiology
Escherichia coli
Gel electrophoresis
Genes
human
Infections
Keywords
Markers
Multidrug resistance
Pathogenicity
Pathogens
PFGE
Pulsed-field gel electrophoresis
Serotypes
Shiga toxin
STEC
Surveillance
Toxins
Virulence
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Summary:Shiga toxin-producing (STEC) isolates (N = 38) that were incriminated in human disease from 2006 to 2013 in South Africa were characterized by serotype, virulence-associated genes, antimicrobial resistance and pulsed-field gel electrophoresis (PFGE). The isolates belonged to 11 O:H serotypes. STEC O26:H11 (24%) was the most frequent serotype associated with human disease, followed by O111:H8 (16%), O157:H7 (13%) and O117:H7 (13%). The majority of isolates were positive for key virulence-associated genes including (84%), (61%), (68.4%) and (55%), but lacked (29%), (42%), (16%), (16%) and (3%). positive isolates carried (26%) and/or (26%) subtypes All pathogenicity island encoded virulence marker genes were detected in all (100%) isolates except (47%), (84%) and (76%). Multidrug resistance was observed in 89% of isolates. PFGE revealed 34 profiles with eight distinct clusters that shared ≥80% intra-serotype similarity, regardless of the year of isolation. In conclusion, STEC isolates that were implicated in human disease between 2006 and 2013 in South Africa were mainly non-O157 strains which possessed virulence genes and markers commonly associated with STEC strains that have been incriminated in mild to severe human disease worldwide. Improved STEC monitoring and surveillance programs are needed in South Africa to control and prevent STEC disease in humans.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins11070424