AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple (Ananas comosus)

Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is po...

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Bibliographic Details
Published in:Horticultural plant journal 2024-03, Vol.10 (2), p.398-412
Main Authors: Zhou, Qiao, Priyadarshani, Samaranayaka Vidana Gamage Nirosha, Qin, Rongjuan, Cheng, Han, Luo, Tiantian, Wai, Myat Hnin, Mohammadi, Mohammad Aqa, Liu, Yanhui, Liu, Chang, Cai, Hanyang, Wang, Xiaomei, Liu, Yeqiang, Qin, Yuan, Wang, Lulu
Format: Article
Language:English
Subjects:
Abiotic stress
AcCPK
AcWRKY
Calcium
ChIP
Chromatin
Coasts
Crop production
Disease resistance
Drought
Economic impact
Fruits
Gene expression
Genes
Genomics
Humidity
Immunoprecipitation
Kinases
Pineapple
Pineapples
Plant growth
Proteins
Rice
Salinity tolerance
Salt
Salt stress
Salt tolerance
Seeds
Transcription activation
Transcription factor
Transcription factors
Transcriptomes
Transgenic plants
Yeasts
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Summary:Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is poorly understood. Here, we studied the pineapple WRKY gene, AcWRKY28, by generating AcWRKY28 over-expressing transgenic pineapple plants. Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that “plant-pathogen interaction” pathway genes, including 9 calcium-dependent protein kinases (CPKs), were up-regulated in AcWRKY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one-hybrid assays revealed AcCPK12, AcCPK3, AcCPK8, AcCPK1, and AcCPK15 as direct targets of AcWRKY28. Consistently, the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt, drought, and disease resistance. This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes.
ISSN:2468-0141
2095-9885
2468-0141
DOI:10.1016/j.hpj.2023.05.002