SIN-3 functions through multi-protein interaction to regulate apoptosis, autophagy, and longevity in Caenorhabditis elegans

SIN3/HDAC is a multi-protein complex that acts as a regulatory unit and functions as a co-repressor/co-activator and a general transcription factor. SIN3 acts as a scaffold in the complex, binding directly to HDAC1/2 and other proteins and plays crucial roles in regulating apoptosis, differentiation...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-06, Vol.12 (1), p.10560-10560, Article 10560
Main Authors: Konwar, Chandrika, Maini, Jayant, Kohli, Surbhi, Brahmachari, Vani, Saluja, Daman
Format: Article
Language:English
Subjects:
631/553/1833
631/553/2711
AKT protein
AKT1 protein
Apoptosis
Autophagy
Caenorhabditis elegans
Cell cycle
Cell differentiation
Cell proliferation
Comparative analysis
Histone deacetylase
Humanities and Social Sciences
Isoforms
Longevity
multidisciplinary
Nematodes
Proteins
Science
Science (multidisciplinary)
Worms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SIN3/HDAC is a multi-protein complex that acts as a regulatory unit and functions as a co-repressor/co-activator and a general transcription factor. SIN3 acts as a scaffold in the complex, binding directly to HDAC1/2 and other proteins and plays crucial roles in regulating apoptosis, differentiation, cell proliferation, development, and cell cycle. However, its exact mechanism of action remains elusive. Using the  Caenorhabditis elegans ( C. elegans ) model, we can surpass the challenges posed by the functional redundancy of SIN3 isoforms. In this regard, we have previously demonstrated the role of SIN-3 in uncoupling autophagy and longevity in C. elegans . In order to understand the mechanism of action of SIN3 in these processes, we carried out a comparative analysis of the SIN3 protein interactome from model organisms of different phyla. We identified conserved, expanded, and contracted gene classes. The C. elegans  SIN-3 interactome -revealed the presence of  well-known proteins, such as DAF-16, SIR-2.1, SGK-1, and AKT-1/2, involved in autophagy, apoptosis, and longevity. Overall, our analyses propose  potential mechanisms by which SIN3 participates in multiple biological processes and their conservation across species and identifies candidate genes for further experimental analysis.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-13864-0