Probing the Hepatitis B Virus E-Antigen with a Nanopore Sensor Based on Collisional Events Analysis

Real-time monitoring, simple operation, and cheaper methods for detecting immunological proteins hold the potential for a solid influence on proteomics and human biology, as they can promote the onset of timely diagnoses and adequate treatment protocols. In this work we present an exploratory study...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors (Basel) 2022-08, Vol.12 (8), p.596
Main Authors: Bucataru, Ioana C, Dragomir, Isabela, Asandei, Alina, Pantazica, Ana-Maria, Ghionescu, Alina, Branza-Nichita, Norica, Park, Yoonkyung, Luchian, Tudor
Format: Article
Language:English
Subjects:
Antibodies
antigen
Antigens
Biosensors
Design and construction
Diagnosis
E coli
Electrolytes
electrophysiology
Hepatitis
Hepatitis associated antigen
Hepatitis B
Hepatitis B e antigen
Immune system
Immunology
Lipids
Measurement
Monoclonal antibodies
monoclonal antibody
nanopore
Nanotechnology
Potassium
Proteins
Proteomics
Sensors
single molecule detection
Steric hindrance
Stochasticity
Subpopulations
Testing
Vestibules
Viral antigens
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Real-time monitoring, simple operation, and cheaper methods for detecting immunological proteins hold the potential for a solid influence on proteomics and human biology, as they can promote the onset of timely diagnoses and adequate treatment protocols. In this work we present an exploratory study suggesting the applicability of resistive-pulse sensing technology in conjunction with the α-hemolysin (α-HL) protein nanopore, for the detection of the chronic hepatitis B virus (HBV) e-antigen (HBeAg). In this approach, the recognition between HBeAg and a purified monoclonal hepatitis B e antibody (Ab(HBeAg)) was detected via transient ionic current spikes generated by partial occlusions of the α-HL nanopore by protein aggregates electrophoretically driven toward the nanopore’s vestibule entrance. Despite the steric hindrance precluding antigen, antibody, or antigen–antibody complex capture inside the nanopore, their stochastic bumping with the nanopore generated clear transient blockade events. The subsequent analysis suggested the detection of protein subpopulations in solution, rendering the approach a potentially valuable label-free platform for the sensitive, submicromolar-scale screening of HBeAg targets.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12080596