A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA

This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticle...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2020-03, Vol.20 (5), p.1509
Main Authors: Wen, Hsin-Yi, Huang, Chun-Wei, Li, Yu-Le, Chen, Jing-Luen, Yeh, Yao-Tsung, Chiang, Chia-Chin
Format: Article
Language:English
Subjects:
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
biosensor
Biosensors
Cell Line, Tumor
Deoxyribonucleic acid
DNA
Equipment Design
Fiber optics
Gold - chemistry
Humans
Immunoglobulins
Limit of Detection
Liver cancer
Metal Nanoparticles - chemistry
MicroRNAs
MicroRNAs - analysis
micrornas detection
Optical Fibers
Self-assembly
Sensors
Silanes
silanization
Silicon Dioxide - chemistry
Spectral sensitivity
Transmission loss
u-shaped optical fiber
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Summary:This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticles and miRNA-133a probe onto the silicon dioxide of the fiber surface. For Mahlavu of hepatocellular carcinoma (HCC), detection is determined by the wavelength shift and transmission loss of a U-shaped optical fiber biosensor. The spectral sensitivity of wavelength and their coefficient of determination are found at -218.319 nm/ ng/mL and 0.839, respectively. Concurrently, the sensitivity of transmission loss and their coefficient of determination are found at 162.394 dB/ ng/mL and 0.984, respectively. A method for estimating the limit of detection of Mahlavu is at 0.0133 ng/mL. The results show that the proposed U-shaped biosensor is highly specific to miRNA-133a and possesses good sensitivity to variations in specimen concentration. As such, it could be of substantial value in microRNA detection.
ISSN:1424-8220
1424-8220
DOI:10.3390/s20051509