Increase in antibiotic resistant Helicobacter pylori in a University Hospital in Japan

Eradication effectively prevents -associated diseases; however, antibiotic resistance has increased throughout Japan and worldwide. This study aimed to assess rates of resistance to antibiotics; amoxicillin, clarithromycin and metronidazole in a University Hospital in Japan. (208 strains) were isola...

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Bibliographic Details
Published in:Infection and drug resistance 2019-03, Vol.12, p.597-602
Main Authors: Kageyama, Chihiro, Sato, Mayu, Sakae, Hiroyuki, Obayashi, Yuka, Kawahara, Yoshiro, Mima, Takehiko, Matsushita, Osamu, Yokota, Kenji, Mizuno, Motowo, Okada, Hiroyuki
Format: Article
Language:English
Subjects:
Amoxicillin
Analysis
Antibacterial agents
Antibiotic resistance
Antibiotics
Antiprotozoan agents
Bacteria
Biopsy
Clarithromycin
Committees
Dentistry
Deoxyribonucleic acid
Diseases
DNA
Drug resistance
Eradication
Gastric cancer
Gene mutation
Genes
Genetic analysis
Genetic testing
genotype
Helicobacter pylori
Hepatology
Hospitals
Metronidazole
Microbial drug resistance
Minimum inhibitory concentration
Mutation
Original Research
Penicillin
Penicillins
Pharmaceutical sciences
Point mutation
Polymerase chain reaction
Polymorphism
Proteins
resistance
Statistical analysis
Strains (organisms)
Surveillance
Systematic review
University hospital
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Summary:Eradication effectively prevents -associated diseases; however, antibiotic resistance has increased throughout Japan and worldwide. This study aimed to assess rates of resistance to antibiotics; amoxicillin, clarithromycin and metronidazole in a University Hospital in Japan. (208 strains) were isolated from patients at the Okayama University Hospital in Japan. The minimum inhibitory concentrations (MIC) were determined using the mean values of the E-test to determine the antimicrobial susceptibilities of the strains. Sequencing and gene analysis were performed to analyze resistance genes to clarithromycin and amoxicillin. Rates of amoxicillin, clarithromycin, and metronidazole resistance were 13%, 48%, and 49%, respectively. Genetic analysis indicated that the A2143G point mutation in 23S rDNA is closely associated with the MIC of clarithromycin. The MIC in amoxicillin-resistant strains increased with an increase in the number of PBP1A amino acids mutations. Genetic analysis for resistant strains is not clinically effective in cases of amoxicillin resistance. Numerous bacteria with already high antibiotic resistance rates have been isolated in large hospitals such as a University Hospital. For effective eradication therapy, MIC measurement should be considered via several methods.
ISSN:1178-6973
1178-6973
DOI:10.2147/idr.s196452