Transfer of oral bacteria to the fetus during late gestation

• Published in: Scientific reports 2021-01, Vol.11 (1), p.708-13, Article 708
• Main Authors: Yu, Kevin, Rodriguez, Michelle, Paul, Zubin, Gordon, Elizabeth, Gu, Tongjun, Rice, Kelly, Triplett, Eric W., Keller-Wood, Maureen, Wood, Charles E.
• Format: Article
• Language: English
• Subjects: 631/443/494; 692/308/3187; Administration, Oral; Animals; Bacteria; Brain - metabolism; Brain - microbiology; Deoxyribonucleic acid; DNA; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Female; Fetus - metabolism; Fetus - microbiology; Fetuses; Fever; Gene expression; Gene Expression Regulation, Bacterial; Gestation; Humanities and Social Sciences; Infectious Disease Transmission, Vertical - statistics & numerical data; Inoculation; Inoculum; Maternal-Fetal Exchange; Mimicry; multidisciplinary; Nutrients; Oral administration; Placenta; Placenta - metabolism; Placenta - microbiology; Pregnancy; RNA-Seq; Science; Science (multidisciplinary); Sepsis; Sheep; Staphylococcal Infections - genetics; Staphylococcal Infections - metabolism; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - isolation & purification
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-80653-y

The fetus develops in a privileged environment, as the placenta serves as both a gateway for nutrients and a barrier for pathogen transfer to the fetus. Regardless, recent evidence suggests the presence of bacterial DNA in both placenta and fetus, and we have reported that DNA and protein from small numbers of bacteria gain access to the fetus from the maternal bloodstream. Other routes of environmental bacterial transfer from the mother to fetus remain unknown, as well as the physiological relevance of their presence. In these experiments, we examine multiple routes by which bacterial cellular components can enter the fetus and the fetal response to influx of bacterial DNA and protein. We inoculated maternal sheep with genetically-labeled  S. aureus  ( Staphylococcus aureus ) using three routes: intravenously, orally, and intra-vaginally. The inoculum did not produce sepsis or fever in the ewes, therefore mimicking incidental exposure to bacteria during pregnancy. 3–5 days post inoculation, we assessed the presence of bacterial components in the fetal tissues and analyzed fetal brain tissue to identify any alterations in gene expression. Our results demonstrate that components of bacteria that were introduced into the maternal mouth were detected in the fetal brain and that they stimulated changes in gene expression. We conclude that an oral route of transmission is relevant for transfer of bacterial cellular components to the fetus.