Comparative analysis of fluorescent methods selectivity for ecotoxicants environmental monitoring

• Published in: Vestnik Rossijskogo universiteta družby narodov. Seriâ Èkologiâ i bezopasnostʹ žiznedeâtelʹnosti (Online) 2022-12, Vol.30 (4), p.574-583
• Main Authors: Plotnikova, Olga A., Tikhomirova, Elena I., Melnikov, Gennadyi V.
• Format: Article
• Language: English
• Subjects: fluorescence analysis; polycyclic aromatic hydrocarbons; simultaneous scanning
• ISSN: 2313-2310; 2408-8919
• DOI: 10.22363/2313-2310-2022-30-4-574-583

A comparative analysis of classical and synchronous fluorescent methods selectivity for ecotoxicants determination of polycyclic aromatic hydrocarbons (PAHs) was carried out. PAHs sorption preconcentration from aqueous cetyltrimethylammonium bromide micellar solutions and spectra recording on a viscose matrix were used. In the case of complex environmental samples, the scope of fluorimetric analysis can be expanded by registering synchronous fluorescence spectra obtained by simultaneously scanning the excitation and emission wavelengths with a constant shift: λem - λex = Δλ = const. The composition of PAHs mixture (phenanthrene, anthracene, fluorene, pyrene) was experimentally evaluated by classical fluorescence with selective excitation and synchronous fluorescence. It was concluded that the use of synchronous spectra makes it possible to improve the selectivity of the PAHs fluorescence analysis in a mixture. These methods can be successfully applied to identify individual PAHs in monitoring of environment water bodies.