Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures

The activation, growth, and maturation of oocytes to an ovulatory phase, termed folliculogenesis, is governed by the orchestrated activity of multiple specialized cell types within the ovary; yet, the mechanisms governing diversification and behavior of discrete cellular sub-populations within folli...

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Bibliographic Details
Published in:Cell reports (Cambridge) 2020-08, Vol.32 (6), p.108027-108027, Article 108027
Main Authors: Man, Limor, Lustgarten-Guahmich, Nicole, Kallinos, Eleni, Redhead-Laconte, Zachary, Liu, Sally, Schattman, Benjamin, Redmond, David, Hancock, Kolbe, Zaninovic, Nikica, Schattman, Glenn, Rosenwaks, Zev, James, Daylon
Format: Article
Language:English
Subjects:
antral follicle
fertility preservation
granulosa cell
human folliculogenesis
ovarian tissue transplantation
ovarian tissue xenograft
single-cell RNA sequencing
theca cell
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Summary:The activation, growth, and maturation of oocytes to an ovulatory phase, termed folliculogenesis, is governed by the orchestrated activity of multiple specialized cell types within the ovary; yet, the mechanisms governing diversification and behavior of discrete cellular sub-populations within follicles are poorly understood. We use bulk and single-cell RNA sequencing to distinguish the transcriptional signature of prospectively isolated granulosa and theca/stroma cell subsets within human antral follicles derived from xenografts or ovaries. The analysis deconstructs phenotypic diversification within small (
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2020.108027