Flow field-flow fractionation coupled with multidetector: A robust approach for the separation and characterization of resistant starch

• Published in: Food Chemistry: X 2024-06, Vol.22, p.101267-101267, Article 101267
• Main Authors: Wang, Mu, Zhang, Wenhui, Yang, Liu, Li, Yueqiu, Zheng, Hailiang, Dou, Haiyang
• Format: Article
• Language: English
• Subjects: Characterization; Flow field-flow fractionation; Resistant starch; Review
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2024.101267

•Classification and structural characterization challenges of RS are discussed.•Theory and variants of flow field-flow fractionation (FlFFF) are introduced.•The controllable factors affecting FlFFF analysis of RS are discussed.•Applications of FlFFF in the structural characterization of RS are reviewed. The unique properties of resistant starch (RS) have made it applicable in the formulation of a broad range of functional foods. The physicochemical properties of RS play a crucial role in its applications. Recently, flow field-flow fractionation (FlFFF) has attracted increasing interest in the separation and characterization of different categories of RS. In this review, an overview of the theory behind FlFFF is introduced, and the controllable factors, including FlFFF channel design, sample separation conditions, and the choice of detector, are discussed in detail. Furthermore, the applications of FlFFF for the separation and characterization of RS at both the granule and molecule levels are critically reviewed. The aim of this review is to equip readers with a fundamental understanding of the theoretical principle of FlFFF and to highlight the potential for expanding the application of RS through the valuable insights gained from FlFFF coupled with multidetector analysis.