Linum lewisii Adventitious and Hairy-Roots Cultures as Lignan Plant Factories

Plants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted fr...

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Bibliographic Details
Published in:Antioxidants 2022-08, Vol.11 (8), p.1526
Main Authors: Dougué Kentsop, Roméo Arago, Consonni, Roberto, Alfieri, Michela, Laura, Marina, Ottolina, Gianluca, Mascheretti, Iride, Mattana, Monica
Format: Article
Language:English
Subjects:
Acids
antioxidant capacity
Antioxidants
Antiviral activity
Bioreactors
Biosynthesis
Cell culture
Cloning
Cytotoxicity
elicitation
flax
Hairy root
Inflammation
justicidin B
lignans
Linum
Magnetic resonance spectroscopy
Metabolism
Metabolites
NMR
Nuclear magnetic resonance
Polyphenols
precursor feeding
Secondary metabolites
Seeds
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Summary:Plants synthesize specific secondary metabolites for survival, reproduction, environmental resilience, and defense. Among them, lignans are a class of polyphenols with several bioactive properties: chemopreventive, anti-inflammatory, antiviral, and antioxidant. These compounds are often extracted from field-grown plants with very low yields. To overcome these constraints, in vitro tissue cultures provide a tool to optimize large-scale production. Moreover, the use of elicitation to increase secondary metabolite production is gaining importance. The aim of this work was to develop adventitious (ARL) and hairy roots (HRL) from Linum lewisi, a species able to synthesize arylnaphthalene lignans such as justicidin B. The ARL and HRL were obtained for the first time and characterized for their phenol content, antioxidant activity, and the production of justicidin B after treatments with several elicitors and precursor feeding. Through NMR spectroscopy, other four lignans were highlighted and identified in the roots extracts. A pilot-scale bioreactor was adopted to assess the suitability of the developed root cultures for future large-scale production. The ARL and HRL cultures showed a justicidin B production higher than other Linum species cultures described up to now (75.8 mg/L and 82.2 g/L), and the production more than doubled after elicitation with MeJA.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox11081526