Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology

A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-03, Vol.24 (7), p.1215
Main Authors: Insuasty Cepeda, Diego Sebastián, Pineda Castañeda, Héctor Manuel, Rodríguez Mayor, Andrea Verónica, García Castañeda, Javier Eduardo, Maldonado Villamil, Mauricio, Fierro Medina, Ricardo, Rivera Monroy, Zuly Jenny
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - metabolism
By products
Chemical synthesis
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Elution
gradient elution
High performance liquid chromatography
Ions
Liquid chromatography
Mathematical models
Methods
peptide
Peptides
Peptides - chemistry
Peptides - isolation & purification
Physicochemical properties
preparative purification
Retention
Retention time
solid phase extraction (SPE)
Solid Phase Extraction - economics
Solid Phase Extraction - methods
solid phase peptide synthesis
Solid phases
Solvents
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Summary:A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24071215