OsWRKY67 Plays a Positive Role in Basal and XA21-Mediated Resistance in Rice

WRKY proteins play important roles in transcriptional reprogramming in plants in response to various stresses including pathogen attack. In this study, we functionally characterized a rice WRKY gene, , whose expression is upregulated against pathogen challenges. Activation of by T-DNA tagging signif...

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Published in:Frontiers in plant science 2018-01, Vol.8, p.2220-2220
Main Authors: Vo, Kieu T X, Kim, Chi-Yeol, Hoang, Trung V, Lee, Sang-Kyu, Shirsekar, Gautam, Seo, Young-Su, Lee, Sang-Won, Wang, Guo-Liang, Jeon, Jong-Seong
Format: Article
Language:English
Subjects:
disease resistance
Magnaporthe oryzae
OsWRKY67
Plant Science
rice
Xanthomonas oryzae pv. oryzae
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Summary:WRKY proteins play important roles in transcriptional reprogramming in plants in response to various stresses including pathogen attack. In this study, we functionally characterized a rice WRKY gene, , whose expression is upregulated against pathogen challenges. Activation of by T-DNA tagging significantly improved the resistance against two rice pathogens, and pv. ( ). Reactive oxygen species (ROS) rapidly accumulated in activation mutant lines in response to elicitor treatment, compared with the controls. Overexpression of in rice confirmed enhanced disease resistance, but led to a restriction of plant growth in transgenic lines with high levels of OsWRKY67 protein. RNAi lines significantly reduced resistance to and isolates tested, and abolished XA21-mediated resistance, implying the possibility of broad-spectrum resistance from OsWRKY67. Transcriptional activity and subcellular localization assays indicated that OsWRKY67 is present in the nucleus where it functions as a transcriptional activator. Quantitative PCR revealed that the pathogenesis-related genes, , and , are upregulated in overexpression lines. Therefore, these results suggest that OsWRKY67 positively regulates basal and XA21-mediated resistance, and is a promising candidate for genetic improvement of disease resistance in rice.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.02220