Ss4368: Pathogen-Associated Molecular Pattern for Inducing Plant Cell Death and Resistance to Phytophthora capsici

• Published in: International journal of molecular sciences 2024-08, Vol.25 (16), p.8674
• Main Authors: He, Ziwen, Peng, Shufang, Yin, Qingqing, Huang, Yuanyuan, Deng, Ting, Luo, Yiwei, He, Ningjia
• Format: Article
• Language: English
• Subjects: Agricultural production; Amino acids; Bacteria; Cell Death; Disease Resistance - genetics; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene Expression Regulation, Plant; Kinases; Nicotiana - genetics; Nicotiana - microbiology; Nicotiana benthamiana; pathogen-associated molecular pattern (PAMP); Pathogen-Associated Molecular Pattern Molecules - metabolism; Pathogens; Peptides; Phytophthora - pathogenicity; Plant Cells - metabolism; Plant Cells - microbiology; Plant Diseases - genetics; Plant Diseases - microbiology; plant immune inducer; Plant Immunity; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Scleromitrula shiraiana
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25168674

Plant recognition of pathogen-associated molecular patterns (PAMPs) is pivotal in triggering immune responses, highlighting their potential as inducers of plant immunity. However, the number of PAMPs identified and applied in such contexts remains limited. In this study, we characterize a novel PAMP, designated Ss4368, which is derived from . Ss4368 is specifically distributed among a few fungal genera, including , , and . The transient expression of Ss4368 elicits cell death in a range of plant species. The signaling peptides, three conserved motifs, and cysteine residues (C46, C88, C112, C130, and C148) within Ss4368 are crucial for inducing robust cell death. Additionally, these signaling peptides are essential for the protein's localization to the apoplast. The cell death induced by Ss4368 and its homologous protein, Bc4368, is independent of the SUPPRESSOR OF BIR1-1 (SOBIR1), BRI1-ASSOCIATED KINASE-1 (BAK1), and salicylic acid (SA) pathways. Furthermore, the immune responses triggered by Ss4368 and Bc4368 significantly enhance the resistance of to . Therefore, we propose that Ss4368, as a novel PAMP, holds the potential for developing strategies to enhance plant resistance against .