Changes in Soil Chemistry and Microbial Communities in Rhizospheres of Planted Gastrodia elata on a Barren Slope and under a Forest

• Published in: Forests 2024-02, Vol.15 (2), p.331
• Main Authors: Xie, Xia, Shi, Rui, Yan, Xinru, Zhang, Ao, Wang, Yonggui, Jiao, Jinlong, Yu, Yang, Horowitz, Abraham Rami, Lu, Jincai, He, Xiahong
• Format: Article
• Language: English
• Subjects: Abundance; Bacteria; Barren lands; Biogeochemistry; Carbon; Chemical properties; Community structure; Composition; Continuous cropping; Cycles; Environmental aspects; Epilepsy; Fungi; Gastrodia elata; Genes; Genomes; Measurement; Metagenomics; Microbial activity; Microbiomes; Microorganisms; mineral element cycling; Next-generation sequencing; Nitrogen; Orchids; Organic carbon; Organic matter; Organic phosphorus; pathogenic microbes; Phosphorus; Phylogenetics; Rhizosphere; Soil analysis; soil chemical properties; Soil chemistry; Soil ecology; Soil improvement; Soil microbiology; soil microecology; Soil nutrients; Soil properties; Soil-borne diseases; Soils; Sulfur; Testing
• ISSN: 1999-4907; 1999-4907
• DOI: 10.3390/f15020331

Continuous cropping of the important achlorophyllous medicinal orchid Gastrodia elata Blume causes an imbalance in soil microecology leading to soil-borne diseases. However, the impacts on different land covers remain largely unknown. Hence, this study aimed to investigate changes in the soil nutrient composition and the global microbial community structure in rhizospheres of G. elata cultivated on a barren slope (HPGJ) and under a forest (LXT) using integrated shotgun metagenomics and an analysis of soil chemical properties. High-throughput sequencing revealed an increase in the abundance of Proteobacteria, Actinobacteria, Mucoromycota, Basidiomycota, and Ascomycota, which drive N- and C-cycling genes in HPGJ and LXT. Notably, the fungal community was significantly improved in the HPGJ (from 0.17% to 23.61%) compared to the LXT (from 0.2% to 2.04%). Consequently, mineral cycling was enhanced in the HPGJ, resulting in a more improved soil nutrient composition than in the LXT. The soil chemical properties analysis unveiled a significant increase in the contents of the total nitrogen, NO3−-N, organic matter, total carbon, organic carbon, total sulfur, and total phosphorus in the HPGJ, while no changes were recorded in the LXT. It was noteworthy that the abundance of pathogenic microorganisms increased significantly in the HPGJ compared to the LXT. Our results provide supporting data to optimize G. elata cultivation on slopes.