Combined effect of DBM, PRP, and bone marrow fluid on bone union in a rat posterolateral fusion model

Platelet-rich plasma (PRP) promotes bone union through osteoinduction. We investigated whether adding demineralized bone matrix (DBM), derived naturally from biomaterial and with various growth factors, for osteoconductivity and bone marrow fluid for osteogenesis results in different bone unions. Ei...

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Bibliographic Details
Published in:Scientific reports 2023-09, Vol.13 (1), p.15041-9, Article 15041
Main Authors: Otagiri, Takuma, Shiga, Yasuhiro, Hozumi, Takashi, Matsuura, Yusuke, Tajiri, Ikuko, Takayama, Naoya, Alejandra, Maria, Shiko, Yuki, Orita, Sumihisa, Inage, Kazuhide, Eguchi, Yawara, Suzuki, Takane, Suzuki-Narita, Miyako, Mukai, Michiaki, Mukaihata, Tomohito, Tsuchiya, Ryuto, Tokeshi, Soichiro, Okuyama, Kohei, Arai, Takahito, Toshi, Noriyasu, Furuya, Takeo, Maki, Satoshi, Aoki, Yasuchika, Ohtori, Seiji
Format: Article
Language:English
Subjects:
631/532
692/308
Biomaterials
Bone biomaterials
Bone growth
Bone imaging
Bone marrow
Bone matrix
Bone strength
Growth factors
Humanities and Social Sciences
multidisciplinary
Osteoconduction
Osteogenesis
Science
Science (multidisciplinary)
Transplantation
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Summary:Platelet-rich plasma (PRP) promotes bone union through osteoinduction. We investigated whether adding demineralized bone matrix (DBM), derived naturally from biomaterial and with various growth factors, for osteoconductivity and bone marrow fluid for osteogenesis results in different bone unions. Eight-week-old male Sprague–Dawley rats were divided into four groups of five based on transplantation material: sham control (C group); DBM alone (D group); DBM + PRP (DP group); and DBM + PRP + bone marrow fluid (DPB group). After posterolateral fusion at L3-5, postoperative weekly CT imaging determined average number of bone union in facet joints (4 joints × 5 animals = 20 joints) and bone formation. Pathological evaluation and bone strength were assessed using 3-point bending two weeks postoperatively. Facet joint bone union at four weeks postoperatively was 4/20 (20%, DP group) and 8/20 (40%, DPB group) joints. Six weeks postoperatively, it was 7/20 (35%, D group), 12/20 (60%, DP group), and 16/20 (80%, DPB group). Eight weeks postoperatively, it was 13/20 (65%, D group), 17/20 (85%, DP group), and 20/20 (100%, DPB group), suggesting that DPB > DP > D > C. Bone formation and bone strength showed a similar DPB > DP > D > C group trend. Adding PRP and bone marrow fluid to DBM promotes bone union and strength.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-41844-5