Improvement in calcified anaerobic granular sludge performance by exogenous acyl-homoserine lactones

• Published in: Ecotoxicology and environmental safety 2021-03, Vol.210, p.111874, Article 111874
• Main Authors: Li, Meiling, Wu, Yueru, Wang, Zhiwei, Fu, Wencai, Dang, Wenhao, Chen, Yongli, Ning, Yi, Wang, Shuangfei
• Format: Article
• Language: English
• Subjects: AHLs; Anaerobic granular sludge; Anaerobiosis; Calcification; Community structure; Extracellular Polymeric Substance Matrix - metabolism; Lactones - administration & dosage; Methane - metabolism; Microbiota - drug effects; Papermaking wastewater; Sewage; Waste Water
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.111874

Given the high content of Ca2+ in waste paper recycling wastewater, the anaerobic granular sludge (AnGS) undergoes calcification during wastewater treatment and affects the treatment efficiency. To restore the activity of calcified AnGS and improve the performance of AnGS, four types of N-acyl-homoserine lactones (AHLs) were added to the AnGS system while papermaking wastewater treatment. The addition of N-butyryl-DL-homoserine lactone(C4-HSL) and N-octanoyl-DL-homoserine lactone (C8-HSL) had an inhibitory affect the COD removal efficiency and SMA of sludge at the inception. The addition of N-hexanoyl-L-homoserine lactone (C6-HSL) has no obvious effect on the COD removal efficiency, but can improve the SMA of sludge more obviously. The addition of N-(β-ketocaproyl)-DL-homoserine lactone (3O-C6-HSL) can increased COD removal efficiency and promoted SMA together obviously. The addition of C6-HSL and 3O-C6-HSL can increase volatile suspended solid (VSS)/total suspended solid (TSS), and regulate extracellular polymeric substance (EPS) secretion in AnGS. Analysis of microbial sequencing revealed changes in the microbial community structure following AHL addition, which enhanced the methane metabolism pathway in sludge. The addition of C6-HSL, C8-HSL, and 3O-C6-HSL increased Methanosaeta population, thus increasing the aceticlastic pathway in sludge. Thus, exogenous AHLs can play an important role in regulating microbial community structure, and in improving the performance of AnGS. •Adding specific AHLs can improve the performance of calcified AnGS.•AHLs can adjust the community structures of bacteria and archaea.•Specific AHLs can enhance the methane metabolism pathway of sludge.