Protective Effects of Fucoidan on Aβ25-35 and d-Gal-Induced Neurotoxicity in PC12 Cells and d-Gal-Induced Cognitive Dysfunction in Mice

• Published in: Marine drugs 2017-03, Vol.15 (3), p.77
• Main Authors: Wei, Hengyun, Gao, Zixiang, Zheng, Luping, Zhang, Cuili, Liu, Zundong, Yang, Yazong, Teng, Hongming, Hou, Lin, Yin, Yuling, Zou, Xiangyang
• Format: Article
• Language: English
• Subjects: Acetylcholine - metabolism; Acetylcholinesterase; Algae; Alzheimer Disease - drug therapy; Alzheimer Disease - metabolism; Alzheimer's disease; Amyloid beta-Peptides - pharmacology; amyloid-β peptide; Animals; Antioxidants - metabolism; Apoptosis; Apoptosis - drug effects; Bioactive compounds; Cell Line, Tumor; Chemical extraction; Choline; Choline O-Acetyltransferase - metabolism; Cognitive Dysfunction - chemically induced; Cognitive Dysfunction - drug therapy; Cognitive Dysfunction - metabolism; Disease Models, Animal; Drugs; Esterases; fucoidan; Galactose - pharmacology; Glutathione - metabolism; Learning; Memory; Mice; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; neuroprotection; Neuroprotective Agents - pharmacology; Neurotoxicity Syndromes - drug therapy; Neurotoxicity Syndromes - metabolism; Older people; Oxidative Stress - drug effects; PC12 Cells; Peptide Fragments - pharmacology; Polysaccharides - pharmacology; Rats; Superoxide Dismutase - metabolism; Toxicity
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md15030077

Alzheimer's disease (AD) is a chronic neurodegenerative disease which contributes to memory loss and cognitive decline in the elderly. Fucoidan, extracted from brown algae, is a complex sulfated polysaccharide and potential bioactive compound. In this study, we investigated whether fucoidan protects PC12 cells from apoptosis induced by a combination of beta-amyloid 25-35 (Aβ25-35) and d-galactose (d-Gal), and improves learning and memory impairment in AD model mice. The results indicated that fucoidan could inhibit the release of cytochrome c from the mitochondria to cytosol and activation of caspases, and increase the expression of apoptosis inhibitor proteins (IAPs), including livin and X-linked IAP (XIAP) in PC12 cells damaged by Aβ25-35 and d-Gal-induction. Fucoidan reversed the decreased activity of acetylcholine (ACh) and choline acetyl transferase (ChAT), as well as the increased activity of acetylcholine esterase (AChE), in AD model mice induced by infusion of d-Gal. Furthermore, fucoidan improved antioxidant activity in vitro and in vivo by activation of superoxide dismutase (SOD) and glutathione (GSH). These results suggested that fucoidan could protect PC12 cells from apoptosis and ameliorate the learning and memory impairment in AD model mice, which appeared to be due to regulating the cholinergic system, reducing oxidative stress, and inhibiting the caspase-dependent apoptosis pathway.