Determinação de hormônios estrógenos em água potável usando CLAE-DAD

An analytical procedure for determination of estriol, 17β-estradiol, estrone and 17α-ethinylestradiol in drinking water is presented. The method employs solid phase extraction (SPE) and sample dechlorination as cleanup procedures, followed by HPLC-DAD analysis. Validation was carried out using RE No...

Full description

Saved in:
Bibliographic Details
Published in:Química Nova 2010, Vol.33 (9), p.1837-1842
Main Authors: Verbinnen, Raphael Teixeira, Nunes, Gilvanda Silva, Vieira, Eny Maria
Format: Article
Language:English
Subjects:
estrogen hormones
HPLC-DAD
water supply
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An analytical procedure for determination of estriol, 17β-estradiol, estrone and 17α-ethinylestradiol in drinking water is presented. The method employs solid phase extraction (SPE) and sample dechlorination as cleanup procedures, followed by HPLC-DAD analysis. Validation was carried out using RE No. 899/2003 guidelines established by the Agência Nacional de Vigilância Sanitária (National Agency of Sanitary Surveillance, Brazil), with some adaptations. The statistically evaluated results have shown that the method is selective, precise (0,06% to 19,40% CV) and accurate (91,52% to 109,41% average recoveries). The developed method was applied to the analysis of these contaminants in drinking water from São Luís, MA.
ISSN:0100-4042
1678-7064
DOI:10.1590/S0100-40422010000900003