CRISPR/Cas9-mediated knockout of SLC15A4 gene involved in the immune response in bovine rumen epithelial cells

The objective of this study was to determine the role of SLC15A4 in the muramyl dipeptide (MDP)-mediated inflammatory response of bovine rumen epithelial cells (BRECs). First, changes in the mRNA expression of pro-inflammatory factor genes in BRECs following 10 μg mL–1 MDP treatments were examined....

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Bibliographic Details
Published in:Journal of Integrative Agriculture 2023-10, Vol.22 (10), p.3148-3158
Main Authors: JIANG, Mao-cheng, HU, Zi-xuan, WANG, Ke-xin, YANG, Tian-yu, LIN, Miao, ZHAN, Kang, ZHAO, Guo-qi
Format: Article
Language:English
Subjects:
CRISPR/Cas9
immune response
MDP
proton-coupled oligopeptide transporter (POT) families
SLC15A4
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Summary:The objective of this study was to determine the role of SLC15A4 in the muramyl dipeptide (MDP)-mediated inflammatory response of bovine rumen epithelial cells (BRECs). First, changes in the mRNA expression of pro-inflammatory factor genes in BRECs following 10 μg mL–1 MDP treatments were examined. RT-qPCR results showed that the expression of pro-inflammatory factor (IL-1β, IL-6, and TNF-α) mRNAs were significantly increased under MDP stimulation (P
ISSN:2095-3119
2352-3425
DOI:10.1016/j.jia.2023.06.016