3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow

Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular mov...

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Bibliographic Details
Published in:IUCrJ 2020-03, Vol.7 (Pt 2), p.207-219
Main Authors: Monteiro, Diana C F, von Stetten, David, Stohrer, Claudia, Sans, Marta, Pearson, Arwen R, Santoni, Gianluca, van der Linden, Peter, Trebbin, Martin
Format: Article
Language:English
Subjects:
3d microfabrication
3D printing
3d-mixd
Chemical Sciences
Consumption
Crystallography
Data collection
Microcrystals
Microfluidic devices
microfluidics
Proteins
Research Papers
Room temperature
serial synchrotron crystallography
structure determination
Synchrotrons
Three dimensional flow
Three dimensional printing
Time
White, T.H. (British writer)
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Summary:Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. This efficient collection scheme in combination with its mixing geometry paves the way for recording molecular movies at synchrotrons by mixing-triggered millisecond time-resolved SSX.
ISSN:2052-2525
2052-2525
DOI:10.1107/s2052252519016865