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3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow
Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular mov...
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Published in: | IUCrJ 2020-03, Vol.7 (Pt 2), p.207-219 |
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creator | Monteiro, Diana C F von Stetten, David Stohrer, Claudia Sans, Marta Pearson, Arwen R Santoni, Gianluca van der Linden, Peter Trebbin, Martin |
description | Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. This efficient collection scheme in combination with its mixing geometry paves the way for recording molecular movies at synchrotrons by mixing-triggered millisecond time-resolved SSX. |
doi_str_mv | 10.1107/s2052252519016865 |
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Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. 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Monteiro et al. 2020.</rights><rights>COPYRIGHT 2020 International Union of Crystallography</rights><rights>2020. This article is published under https://creativecommons.org/licenses/by/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Diana C. F. 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Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. This efficient collection scheme in combination with its mixing geometry paves the way for recording molecular movies at synchrotrons by mixing-triggered millisecond time-resolved SSX.</description><subject>3d microfabrication</subject><subject>3D printing</subject><subject>3d-mixd</subject><subject>Chemical Sciences</subject><subject>Consumption</subject><subject>Crystallography</subject><subject>Data collection</subject><subject>Microcrystals</subject><subject>Microfluidic devices</subject><subject>microfluidics</subject><subject>Proteins</subject><subject>Research Papers</subject><subject>Room temperature</subject><subject>serial synchrotron crystallography</subject><subject>structure determination</subject><subject>Synchrotrons</subject><subject>Three dimensional flow</subject><subject>Three dimensional printing</subject><subject>Time</subject><subject>White, T.H. 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subjects | 3d microfabrication 3D printing 3d-mixd Chemical Sciences Consumption Crystallography Data collection Microcrystals Microfluidic devices microfluidics Proteins Research Papers Room temperature serial synchrotron crystallography structure determination Synchrotrons Three dimensional flow Three dimensional printing Time White, T.H. (British writer) |
title | 3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow |
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