Exosomes secreted from miRNA-29b-modified mesenchymal stem cells repaired spinal cord injury in rats

Exosomes, a kind of extracellular vesicle, are promising therapeutic agents for spinal cord injury (SCI). This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRN...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research 2019-01, Vol.52 (12), p.e8735-e8735
Main Authors: Yu, Tao, Zhao, Cunju, Hou, Shouzhi, Zhou, Weijie, Wang, Baoxin, Chen, Yunzhen
Format: Article
Language:English
Subjects:
Animals
BIOLOGY
BMSCs
Bone marrow
Contractility
Disease Models, Animal
Exosomes
Exosomes - metabolism
Female
GAP-43 protein
Glial fibrillary acidic protein
Health aspects
Immunohistochemistry
Male
MEDICINE, RESEARCH & EXPERIMENTAL
Mesenchymal Stem Cell Transplantation
Mesenchymal stem cells
Mesenchyme
MicroRNA
MicroRNAs - metabolism
miRNA
miRNA-29b
Neurons
Rats
Rats, Sprague-Dawley
Recovery of Function
Regeneration
Reverse Transcriptase Polymerase Chain Reaction
Spinal cord injuries
Spinal Cord Injuries - therapy
Spinal cord injury
Stem cell transplantation
Stem cells
Transfection
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Summary:Exosomes, a kind of extracellular vesicle, are promising therapeutic agents for spinal cord injury (SCI). This article aimed to investigate effects of exosomes secreted from miRNA-29b-modified bone marrow mesenchymal stem cells (BMSCs) on SCI. Exosomes were extracted from BMSCs transfected with miRNA-29b or negative control (miR NC). SCI rats were injected intravenously with exosomes (control exosomes, miRNA-29b exosomes) and BMSCs (miR NC, miRNA-29b) through the tail vein. The expression of miRNA-29b in spinal cord tissues of SCI rats was detected by qRT-PCR. The hind limb motor function was evaluated by Basso Beattie Bresnahan (BBB) score. The histopathological damage and neuronal regeneration in spinal cord tissues was observed by HE staining and immunohistochemistry, respectively. The injection of miRNA-29b exosomes and miRNA-29b BMSCs both significantly increased the expression of miRNA-29b in spinal cord tissues of SCI rats (P
ISSN:0100-879X
1414-431X
1414-431X
1678-4510
DOI:10.1590/1414-431X20198735