Genome-wide analysis of the switchgrass YABBY family and functional characterization of PvYABBY14 in response to ABA and GA stress in Arabidopsis

• Published in: BMC plant biology 2024-02, Vol.24 (1), p.114-114, Article 114
• Main Authors: Wang, Weiwei, Ma, Jiayang, Liu, Hanxi, Wang, Zhulin, Nan, Rui, Zhong, Tao, Sun, Mengyu, Wang, Shaoyu, Yao, Yaxin, Sun, Fengli, Zhang, Chao, Xi, Yajun
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; Bioinformatic analysis; Corn; family; flowering; Functional characterization; Gene expression; Gene Expression Regulation, Plant; Genes; Genes, Plant; Genetic aspects; Genetic transcription; genome-wide association study; Genomes; Genomics; Grasses; Hardiness; Hormone and stress tolerance; Hormones; Identification and classification; Panicum - metabolism; Panicum virgatum; Phylogenetics; Phylogeny; Physiological aspects; Plant growth; Plant Growth Regulators; Plant hormones; Plant Proteins - metabolism; Plant resistance; Plants; Proteins; quantitative polymerase chain reaction; Regulatory mechanisms (biology); Rice; root growth; seed maturation; Sodium chloride; Sorghum; Soybean; Soybeans; Stress (Psychology); Stress, Physiological - genetics; Subgroups; Switchgrass; Synteny; Transcription factors; Transcription Factors - genetics; YABBY family
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-04781-7

The small YABBY plant-specific transcription factor has a prominent role in regulating plant growth progress and responding to abiotic stress. Here, a total of 16 PvYABBYs from switchgrass (Panicum virgatum L.) were identified and classified into four distinct subgroups. Proteins within the same subgroup exhibited similar conserved motifs and gene structures. Synteny analyses indicated that segmental duplication contributed to the expansion of the YABBY gene family in switchgrass and that complex duplication events occurred in rice, maize, soybean, and sorghum. Promoter regions of PvYABBY genes contained numerous cis-elements related to stress responsiveness and plant hormones. Expression profile analysis indicated higher expression levels of many PvYABBY genes during inflorescence development and seed maturation, with lower expression levels during root growth. Real-time quantitative PCR analysis demonstrated the sensitivity of multiple YABBY genes to PEG, NaCl, ABA, and GA treatments. The overexpression of PvYABBY14 in Arabidopsis resulted in increased root length after treatment with GA and ABA compared to wild-type plants. Taken together, our study provides the first genome-wide overview of the YABBY transcription factor family, laying the groundwork for understanding the molecular basis and regulatory mechanisms of PvYABBY14 in response to ABA and GA responses in switchgrass.