Effect of Base Sequence on G-Wire Formation in Solution

The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of prepara...

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Bibliographic Details
Published in:Journal of Nucleic Acids 2010-01, Vol.2010 (2010), p.372-379
Main Authors: Spindler, Lea, Rigler, Martin, Drevenšek-Olenik, Irena, Ma'ani Hessari, Nason, Webba da Silva, Mateus
Format: Article
Language:English
Subjects:
DNA
Gel electrophoresis
Nucleotide sequence
Structure
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Summary:The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating.
ISSN:2090-021X
2090-0201
2090-021X
DOI:10.4061/2010/431651