Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using...

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Bibliographic Details
Published in:eLife 2019-02, Vol.8
Main Authors: Tabor, Kathryn M, Marquart, Gregory D, Hurt, Christopher, Smith, Trevor S, Geoca, Alexandra K, Bhandiwad, Ashwin A, Subedi, Abhignya, Sinclair, Jennifer L, Rose, Hannah M, Polys, Nicholas F, Burgess, Harold A
Format: Article
Language:English
Subjects:
Animal genetic engineering
Animals
Animals, Genetically Modified - genetics
Brain
Brain - physiology
Brain - ultrastructure
brain atlas
Brain Mapping - methods
Brain research
Cell Lineage - genetics
Cre
Danio rerio
DNA-Binding Proteins - genetics
Gal4
Gene Expression Regulation - genetics
Gene mapping
Genetic engineering
imaging
Integrases - genetics
intersectional genetics
Laboratories
Nervous system
Neural networks
Neuroimaging
Neuroimaging - methods
Neurons
Neurons - physiology
Neurons - ultrastructure
Neuroscience
Neurosciences
registration
Tools and Resources
Transcription Factors - genetics
Web browsers
Zebrafish - genetics
Zebrafish - physiology
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Summary:Decoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.42687