MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development

Integrating differential RNA and miRNA expression during neuronal lineage induction of human embryonic stem cells we identified miR-934, a primate-specific miRNA that displays a stage-specific expression pattern during progenitor expansion and early neuron generation. We demonstrate the biological r...

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Bibliographic Details
Published in:eLife 2020-05, Vol.9
Main Authors: Prodromidou, Kanella, Vlachos, Ioannis S, Gaitanou, Maria, Kouroupi, Georgia, Hatzigeorgiou, Artemis G, Matsas, Rebecca
Format: Article
Language:English
Subjects:
Analysis
Axonogenesis
Cell Line
Cell migration
Cloning
Developmental Biology
doublecortin
early neurons
Embryo cells
Embryonic stem cells
Fibroblasts
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genes
Gestation
Human Embryonic Stem Cells - physiology
Humans
Male
Mammals
MicroRNA
MicroRNAs
MicroRNAs - physiology
Microtubule-Associated Proteins - metabolism
miRNA
neural induction
Neural Stem Cells - physiology
neuritogenesis
Neurogenesis
Neurogenesis - genetics
Neurons
Neuropeptides - metabolism
Neurotransmission
Non-coding RNA
PAX6
PAX6 Transcription Factor - metabolism
Primates
progenitor cell
Stem cell transplantation
Stem cells
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Summary:Integrating differential RNA and miRNA expression during neuronal lineage induction of human embryonic stem cells we identified miR-934, a primate-specific miRNA that displays a stage-specific expression pattern during progenitor expansion and early neuron generation. We demonstrate the biological relevance of this finding by comparison with data from early to mid-gestation human cortical tissue. Further we find that miR-934 directly controls progenitor to neuroblast transition and impacts on neurite growth of newborn neurons. In agreement, miR-934 targets are involved in progenitor proliferation and neuronal differentiation whilst miR-934 inhibition results in profound global transcriptome changes associated with neurogenesis, axonogenesis, neuronal migration and neurotransmission. Interestingly, miR-934 inhibition affects the expression of genes associated with the subplate zone, a transient compartment most prominent in primates that emerges during early corticogenesis. Our data suggest that mir-934 is a novel regulator of early human neurogenesis with potential implications for a species-specific evolutionary role in brain function.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.50561