Functional Modulation of Choroid Plexus Epithelial Clusters in Vitro for Tissue Repair Applications

One of the primary obstacles in the restoration or repair of damaged tissues is the temporospatial orchestration of biological and physiological events. Cellular transplantation is an important component of tissue repair as grafted cells can serve as replacement cells or as a source of secreted fact...

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Bibliographic Details
Published in:Cell transplantation 2011-01, Vol.20 (11-12), p.1659-1672
Main Authors: Thanos, C. G., Bintz, B. E., Goddard, Moses, Boekelheide, K., Hall, Susan, Emerich, D. F.
Format: Article
Language:English
Subjects:
Animals
Cell Culture Techniques
Cells, Cultured
Central Nervous System - physiology
Choroid Plexus - cytology
Choroid Plexus - metabolism
Collagen - chemistry
Dermis - physiology
Drug Combinations
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial Cells - transplantation
Gene Expression Regulation
Laminin - chemistry
Lateral Ventricles - cytology
Models, Animal
Prealbumin - genetics
Prealbumin - metabolism
Proteoglycans - chemistry
Regeneration
RNA, Messenger - metabolism
Swine
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
Wound Healing
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Summary:One of the primary obstacles in the restoration or repair of damaged tissues is the temporospatial orchestration of biological and physiological events. Cellular transplantation is an important component of tissue repair as grafted cells can serve as replacement cells or as a source of secreted factors. But few, if any, primary cells can perform more than a single tissue repair function. Epithelial cells, derived from the choroid plexus (CP), are an exception to this rule, as transplanted CP is protective and regenerative in animal models as diverse as CNS degeneration and dermal wound repair. They secrete a myriad of proteins with therapeutic potential as well as matrix and adhesion factors, and contain responsive cytoskeletal components potentially capable of precise manipulation of cellular and extracellular niches. Here we isolated CP from neonatal porcine lateral ventricles and cultured the cells under a variety of conditions to specifically modulate tissue morphology (2D vs. 3D) and protein expression. Using qRT-PCR analysis, transmission electron microscopy, and gene microarray studies we demonstrate a fine level of control over CP epithelial cell clusters opening further opportunities for exploration of the therapeutic potential of this unique tissue source.
ISSN:0963-6897
1555-3892
DOI:10.3727/096368911X564985