Early response of monocyte-derived macrophages from vaccinated and non-vaccinated goats against in vitro infection with Mycobacterium avium subsp. paratuberculosis

• Published in: BMC veterinary research 2021-05, Vol.52 (1), p.69-69, Article 69
• Main Authors: Arteche-Villasol, Noive, Gutiérrez-Expósito, Daniel, Vallejo, Raquel, Espinosa, Jose, Elguezabal, Natalia, Ladero-Auñon, Iraia, Royo, Marcos, Del Carmen Ferreras, María, Benavides, Julio, Pérez, Valentín
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Vaccines - administration & dosage; Calmodulin; Caprine monocyte-derived macrophages; control methods; cost effectiveness; Cytokines; DNA; DNA polymerases; Ethylenediaminetetraacetic acid; fluorescent antibody technique; Gene expression; Glycerol; Goat Diseases - immunology; Goat Diseases - microbiology; Goats; Health aspects; Immune response; Infection; Infections; interleukin-10; interleukin-12; interleukin-17; interleukin-6; Johne's disease; Leukocytes, Mononuclear - immunology; Life Sciences; Lymphocytes; Macrophages; Macrophages - immunology; Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis - immunology; paratuberculosis; Paratuberculosis - immunology; Paratuberculosis - microbiology; Pathogens; Phagocytosis; quantitative polymerase chain reaction; RNA-directed DNA polymerase; Vaccination; Vaccination - veterinary; Vaccines; veterinary medicine; Viability
• ISSN: 1297-9716; 0928-4249; 1746-6148; 1297-9716; 1746-6148
• DOI: 10.1186/s13567-021-00940-y

Paratuberculosis is a disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination is the most cost-effective control method. However, despite the fact that macrophages are the main target cells for this pathogen, the precise mechanisms behind the response of the macrophage to Map infection and how it is modified by vaccination are yet poorly understood. The aim of this study was to investigate the effect of Silirum vaccination in the early immune response of caprine monocyte-derived macrophages (CaMØs). Peripheral blood mononuclear cells (PBMCs) were obtained from vaccinated and non-vaccinated goats, cultured in vitro until differentiation to macrophages and infected with Map. After a 24 h incubation, Map viability and DNA were assessed in culture by viable colony count and real time quantitative polymerase chain reaction (qPCR). In addition, Map phagocytosis and expression of IL-10, IL-12, IFN-γ, TNF-α, IL-17A, IL-1β, iNOS, IL-6 and MIP-1β were also evaluated through immunofluorescence labelling and reverse transcriptase qPCR (RT-qPCR), respectively. A significant reduction of Map viability was observed in both supernatants (P