Hematopoietic Origin of Murine Lung Fibroblasts

Multiple origins, including the bone marrow, have been suggested to contribute to fibroblast populations in the lung. Using bone marrow reconstitution strategies, the present study tested the hypothesis that the bone marrow hematopoietic stem cell (HSC) gives rise to lung tissue fibroblasts in vivo....

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Bibliographic Details
Published in:Stem Cells International 2015-01, Vol.2015 (2015), p.190-200-016
Main Authors: McDonald, Lindsay T., LaRue, Amanda C., Mehrotra, Meenal
Format: Article
Language:English
Subjects:
Bone marrow
Cancer
Collagen
Disease
Fibroblasts
Genotype & phenotype
Growth
Hematopoietic stem cells
Immunoglobulins
Laboratory animals
Population
Pulmonary fibrosis
Stem cells
Studies
Transplants & implants
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Summary:Multiple origins, including the bone marrow, have been suggested to contribute to fibroblast populations in the lung. Using bone marrow reconstitution strategies, the present study tested the hypothesis that the bone marrow hematopoietic stem cell (HSC) gives rise to lung tissue fibroblasts in vivo. Data demonstrate that the nonadherent bone marrow fraction is enriched for CD45+ HSC-derived cells and was able to reconstitute hematopoiesis in lethally irradiated animals. Analysis of peripheral blood and lung tissues from engrafted mice demonstrated the ability of this population to give rise to CD45+/Discoidin-Domain Receptor-2+ (DDR2) circulating fibroblast precursors (CFPs) in blood and fibroblast populations in lung. An HSC origin for lung fibroblasts was confirmed using a novel clonal cell transplantation method in which the bone marrow is reconstituted by a clonal population derived from a single HSC. Together, these findings provide evidence for an HSC contribution to lung fibroblasts and demonstrate a circulating intermediate through the CD45+/DDR2+ HSC-derived CFP.
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2015/159713