Initial Biological Assessment of Upconversion Nanohybrids

Nanoparticles for medical use should be non-cytotoxic and free of bacterial contamination. Upconversion nanoparticles (UCNPs) coated with cucurbit[7]uril (CB[7]) made by combining UCNPs free of oleic acid, here termed bare UCNPs (UC ), and CB[7], i.e., UC@CB[7] nanohybrids, could be used as photoact...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines 2021-10, Vol.9 (10), p.1419
Main Authors: Ferrera-González, Juan, Francés-Soriano, Laura, Galiana-Roselló, Cristina, González-Garcia, Jorge, González-Béjar, María, Fröhlich, Eleonore, Pérez-Prieto, Julia
Format: Article
Language:English
Subjects:
Amino acids
Apoptosis
Calcein
Cancer
Cell death
Confocal microscopy
Contamination
cucurbituril
Cytotoxicity
Endothelial cells
Internalization
L-Lactate dehydrogenase
Lactic acid
Ligands
Nanomaterials
Nanoparticles
Oleic acid
upconversion nanoparticles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nanoparticles for medical use should be non-cytotoxic and free of bacterial contamination. Upconversion nanoparticles (UCNPs) coated with cucurbit[7]uril (CB[7]) made by combining UCNPs free of oleic acid, here termed bare UCNPs (UC ), and CB[7], i.e., UC@CB[7] nanohybrids, could be used as photoactive inorganic-organic hybrid scaffolds for biological applications. UCNPs, in general, are not considered to be highly toxic materials, but the release of fluorides and lanthanides upon their dissolution may cause cytotoxicity. To identify potential adverse effects of the nanoparticles, dehydrogenase activity of endothelial cells, exposed to various concentrations of the UCNPs, was determined. Data were verified by measuring lactate dehydrogenase release as the indicator of loss of plasma membrane integrity, which indicates necrotic cell death. This assay, in combination with calcein AM/Ethidium homodimer-1 staining, identified induction of apoptosis as main mode of cell death for both particles. The data showed that the UCNPs are not cytotoxic to endothelial cells, and the samples did not contain endotoxin contamination. Higher cytotoxicity, however, was seen in HeLa and RAW 264.7 cells. This may be explained by differences in lysosome content and particle uptake rate. Internalization of UC and UC@CB[7] nanohybrids by cells was demonstrated by NIR laser scanning microscopy.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9101419