Single-Cell Transcriptional Profile Construction of Rat Pituitary Glands before and after Sexual Maturation and Identification of Novel Marker Spp1 in Gonadotropes

The mammalian pituitary gland drives highly conserved physiological processes such as somatic cell growth, pubertal transformation, fertility, and metabolism by secreting a variety of hormones. Recently, single-cell transcriptomics techniques have been used in pituitary gland research. However, more...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-05, Vol.25 (9), p.4694
Main Authors: Huang, Qing-Hua, Zhao, Guo-Kun, Wang, Hao-Qi, Wei, Fan-Hao, Zhang, Jin-Yu, Zhang, Jia-Bao, Gao, Fei, Yuan, Bao
Format: Article
Language:English
Subjects:
Adults
Animals
Biomarkers - metabolism
Cells
Female
Follicle Stimulating Hormone - metabolism
Gene expression
Gene Expression Profiling
Gonadotrophs - metabolism
Hormones
Male
Physiology
Pituitary gland
Pituitary Gland - metabolism
Puberty
rat pituitary
Rats
Sexual Maturation - genetics
sexual maturity
Single-Cell Analysis - methods
single-cell RNA sequencing
SPP1
Transcription factors
Transcriptome
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Summary:The mammalian pituitary gland drives highly conserved physiological processes such as somatic cell growth, pubertal transformation, fertility, and metabolism by secreting a variety of hormones. Recently, single-cell transcriptomics techniques have been used in pituitary gland research. However, more studies have focused on adult pituitary gland tissues from different species or different sexes, and no research has yet resolved cellular differences in pituitary gland tissue before and after sexual maturation. Here, we identified a total of 15 cell clusters and constructed single-cell transcriptional profiles of rats before and after sexual maturation. Furthermore, focusing on the gonadotrope cluster, 106 genes were found to be differentially expressed before and after sexual maturation. It was verified that , which is specifically expressed in gonadotrope cells, could serve as a novel marker for this cell cluster and has a promotional effect on the synthesis and secretion of follicle-stimulating hormone. The results provide a new resource for further resolving the regulatory mechanism of pituitary gland development and pituitary hormone synthesis and secretion.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25094694