Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices

The demand for blueberry L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture charact...

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Bibliographic Details
Published in:Plants (Basel) 2020-11, Vol.9 (11), p.1624
Main Authors: Cappai, Francesco, Garcia, Alexandria, Cullen, Ryan, Davis, Matthew, Munoz, Patricio R
Format: Article
Language:English
Subjects:
Antibiotics
Blueberries
blueberry
Cefotaxime
Cultivars
Decontamination
Deoxyribonucleic acid
DNA
Fertilizers
Fluorescence
Genetic transformation
Geneticin
Genotype & phenotype
Glufosinate
Herbicides
Hormones
Hybrids
Kanamycin
Light
light quality
Meristems
Mortality
Paromomycin
Plant growth
plant hormones
plant transformation
Propagation
selection agents
Ticarcillin
Tissue culture
Transformations
Vaccinium corymbosum
Zeatin
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Summary:The demand for blueberry L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1-2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants9111624