Comprehensive Genome Analysis of Colistin-Only-Sensitive KPC-2 and NDM1-1-Coproducing Klebsiella pneumoniae ST11 and Acinetobacter baumannii ST2 From a Critically Ill Patient With COVID-19 in Saudi Arabia

The COVID-19 pandemic has intensified the issue of multidrug-resistant (MDR) infections, particularly in intensive care units (ICUs). This study documents the first known case of coinfection with two extensively drug-resistant (XDR) bacterial isolates in a critically ill patient with COVID-19 in Sau...

Full description

Saved in:
Bibliographic Details
Published in:International journal of microbiology 2024-10, Vol.2024
Main Authors: Al-Zahrani, Ibrahim A, Brek, Thamer M
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics
Antimicrobial agents
Bacteria
Bacterial infections
Bacterial pneumonia
Biofilms
Carbapenemase
Coexistence
Colistin
Coronaviruses
COVID-19
DNA sequencing
Drug resistance
E coli
Gene sequencing
Genes
Genetic testing
Genomes
Genomic analysis
Genomics
Health aspects
Hospitals
Intensive care units
Klebsiella
Laboratories
Multidrug resistance
Nosocomial infections
Nucleotide sequence
Nucleotide sequencing
Pandemics
Pneumonia
Sepsis
Tetracycline
Tetracyclines
Virulence
Whole genome sequencing
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The COVID-19 pandemic has intensified the issue of multidrug-resistant (MDR) infections, particularly in intensive care units (ICUs). This study documents the first known case of coinfection with two extensively drug-resistant (XDR) bacterial isolates in a critically ill patient with COVID-19 in Saudi Arabia. Both XDR isolates were recovered from blood and were resistant to all tested antimicrobial agents except colistin. Whole genome sequencing (WGS) revealed that the K. pneumoniae isolate KP-JZ107 had sequence type 11 (ST11) and core genome MLST (cgMLST 304742), while the A. baumannii isolate AB-JZ67 had ST2 and cgMLST 785. KP-JZ107 was found to possess the virulence plasmid KpVP-type-1, carbapenemase genes bla[sub.NDM] and bla[sub.KPC], and numerous antimicrobial-resistant genes (ARGs). The AB-JZ67 isolate had several biofilm-related genes, including biofilm-associated protein (BAP), csuE, and pgaB, and multiple ARGs, including bla[sub.ADC-25], bla[sub.OXA-23], and bla[sub.OXA-66]. Our findings suggest that the coexistence of KP-JZ107 and AB-JZ67 isolates may indicate their widespread presence in ICUs, requiring comprehensive surveillance studies across all hospitals.
ISSN:1687-918X
1687-9198
DOI:10.1155/2024/9233075