A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)

Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-S...

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Bibliographic Details
Published in:Diagnostics (Basel) 2021-10, Vol.11 (10), p.1838
Main Authors: Peng, Yu-Chi, Cheng, Chia-Hsuan, Yatsuda, Hiromi, Liu, Szu-Heng, Liu, Shih-Jen, Kogai, Takashi, Kuo, Chen-Yen, Wang, Robert Y. L.
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Biosensors
Coronaviruses
COVID-19
Epoxy resins
Genomes
Immunoassay
in vitro diagnostic (IVD)
Nanoparticles
Pandemics
Proteins
Sensors
Severe acute respiratory syndrome coronavirus 2
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
surface acoustic wave (SAW)
Viscosity
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Summary:Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics11101838